麻风溃疡患者外周血CD4^+CD25^+Foxp3^+调节性T细胞水平及临床意义

Level and clinical significance of peripheral blood CD4^+CD25^+Foxp3^+ regulation T cells in patients with leprosy ulcers

目的探讨CD4^+CD25^+Foxp3^+调节性T细胞(Treg)在麻风溃疡发病中的作用。方法选取60例麻风溃疡患者作为试验组,同期匹配麻风病未合并溃疡的患者57例作为对照组及体检健康者68例为健康人组,采用流式细胞仪检测在治疗前(或入组时)、治疗后4周(对照组和健康人组为入组后4周)3组研究对象外周血的CD4^+CD25^+Foxp3^+Treg占CD4^+T细胞的百分比。结果试验组在治疗后4周的CD4^+CD25^+Foxp3^+Treg比例明显低于治疗前,差异有统计学意义(P<0.05),在治疗前和治疗后4周试验组的CD4^+CD25^+Foxp3^+Treg比例均明显高于同时间点的对照组和健康人组,差异均有统计学意义(P<0.05),对照组的CD4^+CD25^+Foxp3^+Treg比例也明显高于健康人组,差异均有统计学意义(P<0.05)。合并复杂性溃疡的麻风患者其外周血的CD4^+CD25^+Foxp3^+Treg比例在治疗前和治疗后均显著高于合并单纯性溃疡的麻风患者,差异均有统计学意义(P<0.05)。结论 CD4^+CD25^+Foxp3^+Treg的表达失衡可能参与了麻风溃疡的发生、发展。

Objective To explore the role of CD4＋ CD25＋ Foxp3＋ regulation T cells in the pathogenesis of leprosy ulcers. Meth- ods Sixty cases of leprosy ulcers were selected as the experimental group,meanwhile 57 matched cases of leprosy without ulcers served as the control group and 68 normal persons undergoing physical examination as the healthy persons group. The levels of CD4＋ CD25＋ Foxp3＋ regulation T cells before treatment and after 4-week treatment and at 4 weeks after entering the group in the healthy persons group were detected by using flow cytometry. Results The proportion of CD4＋ CD25＋ Foxp3＋ Treg after 4-week treatment in the experimental group was significantly lower than that before treatment, and the difference was statistically signifi- cant（P〈0.05）. The proportion of CD4＋ CD25＋ Foxp3＋ Treg before and after 4-week treatment in the experimental group was sig- nificantly higher than that at the same time point in the control group and healthy persons group, and the difference were statistical- ly signifieant（P〈0.05）. The proportion of CD4＋ CD25＋ Foxp3＋ Treg before and after treatment in the patients with leprosy com- plicating complex ulcers were significantly higher than that in the patients with leprosy complicating simple ulcer, and the difference were statistically significant（P〈0.05）. Conclusion The expression imbalance of CD4＋ CD25＋ Foxp3＋ T lymphocytes may be in- volved in the occurrence and development of leprosy ulcer.