靶向性紫杉醇纳米胶束的制备及体外评价

Preparation of Paclitaxel-loaded Nanomicells and in Vitro Characteristics

为了增加紫杉醇溶解度和稳定性,采用薄膜分散法制备紫杉醇纳米胶束;采用粒径测定仪测定粒径和PDI;采用UV法测定药物的含量,计算载药量和包封率;采用膜透析法对载药聚合物胶束的体外释药进行考察。本研究制备的纳米胶束粒径分布均匀,平均粒径为(64.34±1.83)nm,包封率大于85%;紫杉醇纳米胶束体外释放显示了良好的缓释特性。本研究制备的紫杉醇纳米胶束制备工艺操作简单,制备得到载药胶束的粒径较小且分布均匀,包封率、载药量较高。

To obtain paclitaxel-loaded nanomicells （FT-PTX-PMs） and assess their characters in vitro, the FT- PTX-PMs were. prepared by film dispersion method. The FT-PTX-PMs were measured by dynamic light scattering. The release curve was determined by dialysis. The content of paclitaxel were determined by UV. The FF-PTX-PMs had a mean particle size of （64. 34±1.83） nm with PDI 0. 089±0. 011. The entrapment efficiency and drug loading of FI＇-PTX- PMs were above 85%. Release of FT-PTX-PMs exhibited sustained release effect. The prepared FT-PTX-PMs exhibited sustained release in vitro, and the particle size, encapsulation efficiency and drug loading were controllable.