miR-346在鼻咽癌中的表达及生物学功能

Expression and biological functions of miR-346 in nasopharyngeal carcinoma

目的:探讨miR-346在鼻咽癌中的表达及在鼻咽癌中的生物学功能。方法:收集63例鼻咽癌组织以及34例鼻咽部非癌组织标本,Real-time PCR检测组织和6株人鼻咽癌细胞系及1株人正常鼻咽部永生化上皮细胞株NP69中miR-346表达量,选取人鼻咽癌细胞系中表达量居中的两种细胞系,并转染miR-346 inhibitor,设置对照组(NC组)并转染阴性对照质粒,Real-time PCR检测两种细胞系转染miR-346 inhibitor和对照组miR-346表达量,细胞增殖、凋亡分别采用Brdu-ELISA和流式细胞术检测,Transwell小室实验检测细胞迁移和侵袭。结果:与鼻咽非癌组织比较,鼻咽癌组织中miR-346表达量显著升高(P=0.000);以正常人鼻咽部上皮细胞NP69比较,各鼻咽癌细胞株中miR-346相对表达量均显著升高,差异有统计学意义(P<0.05)。选择6种鼻咽癌细胞株中miR-346表达量居中的两种,分别为CNE-1和CNE-2,转染miR-346 inhibitor后,两种鼻咽癌细胞株miR-346相对表达量均显著降低,与NC组比较差异均有统计学意义(P<0.05)。两种鼻咽癌细胞转染miR-346inhibitor下调miR-346表达后鼻咽癌细胞的增殖受到抑制,均在转染第3天差异具有统计学意义(P<0.05)。下调miR-346表达后鼻咽癌细胞CNE-1和CNE-2的凋亡显著增加,迁移细胞数和侵袭细胞数显著降低,与NC组比较差异均有统计学意义(P<0.05)。结论:miR-346在鼻咽癌中高表达,下调miR-346表达会抑制鼻咽癌细胞增殖、迁移和侵袭,促进凋亡,miR-346可能作为一种癌基因在鼻咽癌的发生和发展中发挥重要的作用。

Objective ：To explore the expression and biological functions of miR-346 in nasopharyngeal carcinoma. Methods： 63 cases nasopharyngeal carcinoma tissue and 34 cases nasopharyngeal non-cancer tissues were collected,the miR-346 expression were detected by Real-time PCR between nasopharyngeal carcinoma tissue and nasopharyngeal non-cancer tissues ,6 strains of human naso- pharyngeal carcinoma cell lines and 1 strain of normal nasopharyngeal epithelial ceil immortalized NP69. Two cell lines with middle ex- pression levels in human nasopharyngeal carcinoma cells lines were selected, and transfected into miR-346 inhibitor, the control group （NC group） were with negative control plasmid transfection, miR-346 expression in two groups were detected by Real-time PCR, the proliferation,apoptosis were detected by Brdu-ELISA and flow cytometry, the migration and invasion were detected by Transwell Chambers experiments. Results： Compared with the nasopharyngeal non-cancer tissues, the miR-346 expression in nasopharyngeal carcinoma tissues was significantly increased （ P = 0. 000） ; compared with the normal nasopharyngeal epithelial cells NP69. the miR-346 expression in human nasopharyngeal carcinoma cell lines was significantly increased （P〈0. 05 ）. The CNE-1 and CNE-2 were chose, after the miR-346 inhibitor transfection, the miR-346 expression were significantly lower compared with NC group, the difference was statistically significant （P〈0. 05 ）. The proliferation of two kinds of nasopharyngeal carcinoma cell CNE-1 and CNE-2 were restrained after transfection, the difference showed statistically significant 3 days after transfection （P 〈 0. 05 ）. The apoptosis increased significantly, and the migration cell numbers and invasion cell numbers decreased significantly compared with NC group, the differences were statistically significant（ P〈0. 05 ）. Conclusion： miR-346 is overexpression in nasopharyngeal carcinoma, down-regn-lation of miR- 346 inhibits the proliferation, migration, invasion, promotes the apoptosis, miR-346 may act as a oncogene and play an important role in the pathogenesis of nasopharyngeal carcinoma.