α-硫辛酸对高糖环境下大鼠INS-1细胞的保护作用

Protective Effect of Alpha-lipoic Acid on INS-1 Cells of Rats Cultured with High Glucose

目的观察α-硫辛酸对高糖环境下INS-1细胞的抗氧化作用及对胰岛素分泌的影响,探讨α-硫辛酸对胰岛β细胞的保护作用。方法 INS-1细胞分为3组:对照组(11.1 mmol/L葡萄糖处理)、高糖组(30 mmol/L葡萄糖处理)和观察组(30 mmol/L葡萄糖+200μmol/Lα-硫辛酸处理)。分组培养48 h后检测活性氧生成的情况,行葡萄糖刺激胰岛素分泌试验(GSIS)测定胰岛素含量,测定各组氧化应激相关指标,测定核因子E2相关因子2(Nrf2)、血红素加氧酶1(HO-1)、醌氧化还原酶1(NQO1)mRNA表达情况。结果高糖组细胞存活率、超氧化物歧化酶(SOD)、谷胱甘肽过氧化酶1(GPX-1)和过氧化氢酶(CAT)含量低于对照组(P<0.05),活性氧和丙二醛含量高于对照组(P<0.05)。观察组细胞存活率、SOD、GPX-1和CAT含量高于高糖组(P<0.05),活性氧和丙二醛含量低于高糖组(P<0.05)。高糖组基础胰岛素分泌和GSIS试验胰岛素分泌量以及SOD、GPX-1、CAT、HO-1和NQO1 mRNA表达低于对照组(P<0.05)。观察组基础胰岛素分泌和GSIS试验胰岛素分泌量以及SOD、GPX-1、CAT、Nrf2、HO-1和NQO1mRNA表达高于高糖组(P<0.05)。结论α-硫辛酸显著降低胰岛β细胞在高糖环境下的氧化损伤,增强胰岛β细胞GSIS的功能,对胰岛β细胞具有保护作用。

Objective To observe effects of α-lipoic acid on anti-oxidation of INS-1 cells and insulin secretion under high glucose environment, and to investigate protective effect of α-lipoic acid on pancreatic β cells. Methods INS-1 cells were divided into control group （11.1 mmol/L glucose treatment） , high glucose group （30 mmol/L glucose treatment） and observation group （30 mmol/L glucose ＋ 200 μmol/L α-lipoic acid treatment）. After 48 h of cultiva- tion, production conditions of reactive oxygen were detected, glucose-stimulated insulin secretion （GSIS） assay was per- formed to detect insulin concentration. Related indexes of oxidative stress, and mRNA expressions of nuclear factor E2- related factor 2 （ Nrf2）, heme oxygenase-1 （HO-1）, NAD （P） H quinone oxidoreductase （NQO1） were also detected. Results In high glucose group, values of cell survival rate, and contents of superoxide dismutase （ SOD ） , glutathione peroxidase 1 （GPX-1） and catalase （CAT） were significantly lower （P 〈 0. 05 ） , while reactive oxygen species and malondialdehyde contents were significantly higher than those in control group （P 〈 0.05）. In observation group, values of cell survival rate, SOD, GPX-1 and CAT contents were significantly higher （P 〈 0. 05） , while reactive oxygen species and malondialdehyde contents were significantly lower than those in high glucose group （P 〈 0. 05 ）. Values of basal insu- lin secretion, insulin secretion in GSIS assay, and mRNA expressions of SOD, GPX-1, CAT, HO-1 and NQO1 in high glucose group were significantly lower than those in control group （P 〈 0. 05）. Values of basal insulin secretion, insulin secretion in GSIS assay, and mRNA expressions of SOD, GPX-1, CAT, Nrf2, HO-1 and NQO1 in observation group were significantly higher than those in high glucose group（ P 〈 0.05 ）. Conclusion The α-lipoic acid can significantly reduce oxidative damage of islet 13 cells under high glucose environment and enhance GSIS function of pancreatic 13 cells, and it has protective effect on pancreatic 13 ceils.