Fis介导伤寒沙门菌滞留菌形成

Effect of Fis on the persistence of Salmonella enterica serovar Typhi

目的:探讨伤寒沙门菌滞留现象的存在,研究Fis在伤寒沙门菌滞留菌形成中发挥的作用。方法:用药敏纸片和最小抑菌浓度检测进行药敏实验,挑选伤寒沙门菌敏感的抗生素。利用菌落计数法,绘制细菌存活曲线。qRT-PCR检测伤寒沙门菌fis的表达。采用细菌滞留实验分析细菌滞留能力。结果:伤寒沙门菌在对数生长的早期和中晚期都存在滞留现象。与野生株相比,fis基因缺失株的滞留能力下降。结论:Fis可参与伤寒沙门菌滞留菌的形成。

Objective：To investigate whether Salmonella enterica serovar Typhi exsits persistence and the role of Fis in persistence of S.Typhi.Methods：Drug sensitive paper and minimum inhibitory concentration（MIC） test were utilized to select the appropriate antibiotics for detecting the persisters of S.Typhi.Colony-Forming Units （CFU） which counted at various time points were used to draw the bacterial survival curve.The expression of fis in S.Typhi was tested by qRT-PCR.Persistence test was used to analyze the bacterial ability of persitence formation.Results：The persistence of S.Typhi could be detected in the early and mid-late log phase.Compared with the wild type strain, the fis deleted mutant had a decrease in persistence.Conclusion：Fis is involved in forming persisters of S.Typhi.