稻瘟病菌机械敏感性离子通道的鉴定及功能分析

Identification and Functional Analysis of Mechanosensitive Channels in Magnaporthe oryzae

稻瘟病是水稻上危害最为严重的病害之一,其病原菌为子囊菌门稻瘟病菌。作为模式真菌,研究稻瘟病菌的致病机理不仅能为其病害防治提供理论依据,也给其它病原真菌提供借鉴意义。离子通道是细胞内外物质交换的窗口,具有成为防治靶标的潜力,对病害防控具有重要意义。机械敏感性离子通道是一类通过感受细胞膜张力变化来实现胞内外机械信号转导的离子通道。运用生物信息学鉴定了稻瘟病菌的3条机械敏感性离子通道蛋白Mo Msc1,Mo Mid1和Mo Yvc1,进化分析表明它们在真菌里具有保守性。还对稻瘟病菌的小电导率机械敏感性离子通道蛋白Mo Msc1进行了功能分析,该基因沉默并未影响其生长发育、外界胁迫、细胞壁完整性及致病力。

Rice blast disease,caused by ascomycete Magnaporthe oryzae,is one of the most devastating diseases threatening riceproduction. Understanding its pathogenic mechanism not only provides strategies for disease control,but also generates new knowledge offungal pathogenesis. Mechanosensitive channels,the window of material exchange,can be the biocontrol targets for disease control. Thesechannels can sense changes of membrane tension and transduce this signal into intracellular environment. In this study,we identified threemechanosensitive channels（Mo Msc1,Mo Mid1,and Mo Yvc1）in M. oryzae by bioinformatics method. The results showed that the three geneswere evolutionarily conserved in fungi. Furthermore,we discovered that silence of Mo Msc1,encoding the putative gene of mechanosensitivechannel of small conductance in M. oryzae,did not affect its vegetative growth,asexual development,stress response,cell wall integrity andvirulence.