四种新型农药对斑马鱼胚胎发育的毒性效应

Toxic Effects of Four Currently-used Pesticides on Zebrafish Embryonic Development

为了研究4种农药(腈苯唑、吡唑醚菌酯、恶霜灵、戊菌隆)单独及联合作用对斑马鱼胚胎发育的影响,设计7个实验组(空白组、溶剂组、4种农药单独暴露组及联合暴露组)对斑马鱼胚胎暴露72 h,结果显示,浓度50 ng/L的4种农药单独暴露导致心包囊和卵黄囊水肿比率、脊柱弯曲率均显著高于对照组;腈苯唑、恶霜灵及联合暴露导致斑马鱼仔鱼心率显著下降,出现显著的心律不齐;斑马鱼胚胎的Na^+/K^+-ATP酶和Ca^(2+)-ATP酶活性被显著诱导,而联合暴露表现出显著的抑制作用。结果说明,环境浓度的农药暴露对鱼类胚胎发育具有明显的毒性。

In order to study the effects of four pesticides（fenbuconazole,pyraclostrobin,metoxazon,and pencycurone）,eitherindividually or jointly,on the development of zebrafish embryos,seven experiments（blank control,solvent control,individual of 4pesticides,and jointly combined four pesticides）were designed to treat zebrafish embryos for 72 h. The results showed that individualexposure to any of 4 pesticides at 50 ng/L led to the significant elevation on the rate of pericardial and yolk sac edema and the frequency ofdorsal curvature in the larvae compared to the control. The exposure to fenbuconazole,metoxazon,and combined four pesticides resulted ina significant reduction in the heart rate and a significant increase in cardiac arrhythmia in the zebrafish larvae. The activities of Na~＋/K~＋-ATPaseand Ca^（2＋）-ATPase of the embryos were significantly induced by the individual exposure to 4 pesticides,while the both activities were remarkablyinhibited by the combined exposure. The results confirmed that these pesticides at environmental relevant concentration presented the cleartoxicity to the development of fish embryo.