雌酮胁迫农田土壤微生物群落结构变化研究

Study on the Changes of Soil Microbial Community Structure in Farmland by Estrone Stress

雌酮是具有雌效应的天然类固醇雌激素之一,具有高毒性、难降解的特性,可通过食物链蓄积于体内,威胁人类和动物的健康。使用PCR-DGGE技术分析雌酮胁迫下农田土壤微生物群落结构变化。结果表明,雌酮浓度越高,对微生物种群的影响越深,种群的亲缘关系也就越复杂。在雌酮浓度为500 ng/kg至2 000 ng/kg时,微生物群落多样性随着E1浓度的增加而减小,超过某一值时其多样性则会迅速增加,继续增大E1浓度,多样性增加趋势则逐渐变缓。雌酮在微生物作用下可以转变为雌二醇、雌三醇和结合态的E1-3G,这种相互转变也会给微生物菌群多样性带来更加复杂的变化。

Estrone is one of natural steroidal estrogens which have female effect and characterizes by high toxicity and refractory.It can accumulate in the body through the food chain,threatening human and animal health. In this study,PCRand DGGE was used toanalyze the variation of the microbial community structure in estrone-contaminated farmland soil samples. The results showed that the higherthe concentration of estrone,the deeper the effect on the microbial population,and the more complex the phylogenetic relationship amongpopulations. When the concentration of estrone was 500 ng/kg to 2 000 ng/kg,the diversity of microbial community decreased with theincrease of E1 concentration;the diversity increased rapidly when the concentration of estrone was over a certain value,and the increasetrend of diversity gradually became slower when the concentrations of E1 continuously increased. Estrone in the presence of microorganismscan be transformed to estradiol,estriol and bound-state E1-3G,this transformation brings more complex changes to the microbial communitydiversity.