远端上游元件结合蛋白1在胃癌中的表达及其对胃癌细胞增殖和侵袭能力的影响

Expression of Far Upstream Element Binding Protein 1 in Gastric Cancer and Its Effect on Proliferation and Invasion of Gastric Cancer Cells

目的研究远端上游元件结合蛋白1(FUBP1)在胃癌中的表达,探讨其对胃癌细胞增殖侵袭能力的影响。方法采用实时PCR检测FUBP1基因在胃癌组织中的表达。采用单因素方差分析分析FUBP1基因表达与胃癌临床病理因素的相关性。构建FUBP1基因表达沉默载体p S-FUBP1并转染SGC7901细胞,实时PCR检测转染效果。采用CCK8法及Transwell法检测FUBP1基因表达沉默对于SGC7901细胞的增殖和侵袭能力的影响。结果 FUBP1基因在胃癌组织中表达显著上调,且随着分化水平的降低、浸润深度和转移淋巴结数量的增加,胃原发癌中FUBP1基因表达明显增高。p S-FUBP1载体能够显著沉默FUBP1基因的表达。FUBP1表达沉默的SGC7901细胞的增殖及侵袭能力下调明显。结论 FUBP1基因在胃癌中高表达,与胃癌的发生进展相关,沉默其表达能够抑制胃癌细胞的增殖和侵袭能力。

Objective To investigate the expression of far upstream element binding protein 1 （FUBP1） gene in gastric cancer, and analyze its effect on proliferation and invasion of gastricc cancer cells. Methods The expression of FUBP1 in gastric cancer tissues was detected by real-time PCR. The relationship between FUBP1 expression and clinic pathological factors of gastricc cancer was analyzed by one-way ANOVA. The silence vector for FUBPI was constructed and transfected into gastricc cancer SGC7901 cells, and the transfected effects were then detected by real-time PCR. The influence of FUBP1 silence on cell proliferation and invasion in SGC7901 cells were detected by CCK8 method and Transwell assay. Resuits FUBP1 expression was increased markedly in gastricc cancer tissues. Following the depressing of differentiation and the increase of invasion depth and metastatic lymph nodes, the FUBP1 expression was up-regulated in primary gastric carcinoma significantly. The pS-FUBP1 vector could silence the FUBPI expression in SGC7901 cells. FUBP1 silence inhibited SGC7901 cell proliferation anld invasion. Conclusion FUBP1 was high-expressed in gastricc cancer, and related with genesis and progress of gastric cancer, silence of FUBP1 expression can inhibit the cell proliferation and invasion in gastric cancer cells.