Tim-1蛋白表达及其基因多态性与系统性红斑狼疮患者营养功能的相关性

Association of Tim-1 Protein Expression and Gene Polymorphism with Nutritional Function in Patients with Systemic Lupus Erythematosus

目的探讨Tim-1蛋白表达及其基因多态性与系统性红斑狼疮(SLE)患者营养功能指标的相关性。方法收集126例SLE患者外周血样;采用ELISA法检测血清中人Tim-1蛋白水平;采用PCR-RFLP法检测外周血DNA样本Tim-1基因-416G>C和-1454G>A多态性;应用免疫比浊法检测血清标本中前白蛋白、铜蓝蛋白、视黄醇结合蛋白的水平;采用电化学发光法检测血清标本中铁蛋白、1,25-二羟维生素D3的水平。结果血清Tim-1蛋白、前白蛋白、铜蓝蛋白、视黄醇结合蛋白、铁蛋白及1,25-二羟维生素D3的浓度分别为(249.7±30.2)pg/m L、(226±42)μg/m L、(363±95)μg/m L、(29.4±13.2)μg/m L、(355±164)ng/m L、(26.4±11.5)ng/m L;-416G>C位点上GG基因型占11.9%,GC基因型占57.1%,CC基因型占31.0%;-1454G>A位点上GG基因型占67.5%,GA基因型占26.2%,AA基因型占6.3%。-416G>C位点不同基因型之间(F=0.575,P=0.564)及-1454G>A位点不同基因型之间(F=1.255,P=0.289)Tim-1蛋白浓度均无统计学差异。Tim-1与前白蛋白显著负相关(r=-0.176,P=0.033),与铜蓝蛋白(r=0.205,P=0.014)和1,25-二羟维生素D3(r=0.166,P=0.042)均显著正相关。-1454G>A位点GG基因型患者血清中前白蛋白水平显著降低(P=0.027),AA基因型患者血清中1,25-二羟维生素D3水平显著降低(P=0.024)。结论血清Tim-1蛋白水平及Tim-1基因-1454G>A位点多态性与SLE患者营养功能指标存在相关性。

Objective To investigate the association of Tim- 1 protein expression and its gene polymorphism with nutritional parameters in patients with systemic lupus erythematosus （SLE）. Methods Peripheral blood samples were collected from 126 patients with SLE. Serum Tim-1 protein levels were detected by ELISA, and the Tim-1 gene -416G〉C ,-1454G〉A polymorphism was detected by PCR-RFLP. Prealbumin, cemloplasmin, and retinol conjugated protein levels were determined by immunoturbidimetry. Ferritin and 1,25-dihydroxy vitamin D3 levels were detected by electrochemiluminescence. Results Concentrations of serum Tim- 1 protein, prealbumin, ceruloplasmin, retinol binding protein, ferritin, and 1,25-dihydroxy vitamin D3 were 249.7±30.2 pg/mL, 226＋42 μg/mL,363±95 μg/mL,29.4±13.2 μg/mL,355±164 ng/mL, and 26.4±11.5 ng/mL, respectively. In the -416G〉C site, GG, GC, and CC genotypes accounted for 11.9%, 57.1%, and 31.0%, respectively. In the - 1454G〉A site, GG, GA, and AA genotypes accounted for 67.5%, 26.2%, and 6.3%, respectively. The Tim-1 protein concentration did not differ significantly between the different genotypes of the -416G〉C site （F= 0.575 ,P= 0.564 ） or -1454G〉A site （F= 1.255,P= 0.289 ）. Tim-1 level was significantly negatively correlated with prealbumin （r = -0.176, P = 0.033 ）, and positively correlated with eeruloplasmin （r = 0.205, P = 0.014 ） and 1,25-dihydroxy vitamin D3 （r = 0.166, P = 0.042 ）. The serum prealbumin level decreased significantly （P = 0.027 ） in patients harboring the GG genotype in the -1454G〉A site, whereas the serum 1,25-dihydroxy vitamin D3 level decreased significantly （P = 0.024 ） in patients with the AA genotype in the -1454G〉A site. Conclusion Serum Tim-1 protein level and the -1454G〉A polymorphism of Tim-1 gene are associated with the nutritional function of patients with SLE.