三阴乳腺癌中CXCR1和CXCR2的表达及临床意义

Expression and Clinical Significance of CXCR1 and CXCR2 in Triple-negative Breast Cancer

目的探讨CXCR1和CXCR2蛋白在三阴乳腺癌(TNBC)组织中的表达及意义。方法收集乳腺癌(BC)癌旁正常组织28例,乳腺非特殊型浸润性导管癌(IDC-NOS)30例,TNBC组织34例,应用免疫组化En Vision法检测CXCR1和CXCR2蛋白的表达,并分析其与患者临床病理特征的关系,以及CXCR1和CXCR2蛋白与TNBC的相关性。结果在TNBC中,CXCR1和CXCR2蛋白表达的阳性率(23.5%,35.3%)明显低于IDC-NOS(63.3%,60.0%)和BC癌旁正常组织(89.3%,92.9%)。在TNBC中,CXCR1和CXCR2蛋白的表达与临床病理特征(年龄、淋巴结转移、肿瘤大小、组织学分级)无相关性(P>0.05)。CXCR1和CXCR2蛋白的表达水平呈显著正相关(r=0.606,P<0.01)。结论 CXCR1和CXCR2蛋白在BC癌旁正常组织、IDC-NOS和TNBC中均有表达,呈强阳性、中度阳性、阴性递减,且二者在TNBC中的表达呈显著正相关。CXCR1和CXCR2蛋白的表达与TNBC的发生、发展密切相关。

Objective To investigate the expression of CXC chemokine receptor 1 （CXCR1） and CXC chemokine receptor 2 （CXCR2） in triplenegative breast cancer （TNBC） and its significance. Methods The EnVision immunohistochemistry system was used to detect the expression of CXCR1 and CXCR2 in 28 specimens from normal tissues adjacent to breast cancer, 30 specimens of invasive ductal carcinoma not otherwise speci- fied （ IDC-NOS ）, and 34 TNBC specimens. The relationship between the expression of CXCR 1 and CXCR2, and clinical pathological features was analyzed. Results In the adjacent normal tissues of breast cancer, the positive expression rates of CXCR 1 and CXCR2 were 89.3% and 92.9%, respectively ; CXCR1 and CXCR2 expression displayed no significant association with clinicopathological parameters such as age, tumor size, or Searff-Bloom-Richardson （SBR） score. The positive expression rates of CXCR1 and CXCR2 were 63.3% and 60.0%, respectively, in the IDC- NOS samples and 23.5% and 35.3%, respectively, in the TNBC samples.There was a positive correlation between CXCR1 and CXCR2 expressian in the TNBC samples （r = 0.606, P 〈 0.001 ）. Conclusion Correlation of CXCR1 and CXCR2 expression with sample type was strong in adjacent normal tissues, moderate in IDC-NOS samples, and low in TNBC samples. CXCR1 expression was positively correlated with CXCR2 expression in all sample, types. The expression of CXCR1 or CXCR2 was closely related to the development and promotion of TNBC.