摘要
以‘石硖’龙眼为试材,采用RT-PCR结合RACE技术成功克隆一个龙眼漆酶基因全长c DNA序列,命名为Dl Lac,NCBI登录号为KY051551。Dl Lac基因序列全长1898 bp,编码576个氨基酸,NCBI比对结果显示其与荔枝漆酶氨基酸序列同源性最高,高达94%;进化树结果显示龙眼漆酶氨基酸序列与荔枝漆酶氨基酸序列具有高度同源性。氨基酸保守序列结果表明龙眼漆酶氨基酸序列含有漆酶的3个典型保守结构域,分别为:Cu-oxidase-3、Cu-oxidase和Cu-oxidase-2。结合龙眼果实在常温、低温贮藏条件下,果皮褐变与Dl Lac的表达关系,推测Dl Lac的上调表达可能对龙眼果皮褐变起促进作用。
In the present study, laccase gene cDNA was cloned and sequenced from ‘shixia＇ longan cultivar using RT-PCR and RACE,which was named as DlLac. The NCBI nmnbers were KY051551. The DlLac gene consists of 1898 bp encoding a polypeptide of 576 amino aeids.DlLac gene had the highest homology with the Litchi chinensis（94% ）through the NCBI blastn. Amino acids of laccase gene sequence had 3 highly conservative domain structure：Cu-oxidase-3, Cu-oxidase and Cu-oxidase-2. During the normal and cold storage, combining the relationship of pericarp browning and D1Lac expression, it suggested that the laccase gene in pericarp may contribute to the postharvest longan Deriearp browning.
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第13期95-100,共6页
Science and Technology of Food Industry
基金
国家现代农业产业技术体系(荔枝龙眼)(CARS-33-14)资助
贺州学院博士启动基金(HZUBS201510)
贺州市科技开发项目(贺科攻1541008)
广西中青年教师基础能力提升项目(KY2016YB457)
广西特聘专家专项经费
广西果蔬保鲜和深加工研究人才小高地开放课题(2016XGDSHFW02)
关键词
龙眼
漆酶
果皮褐变
基因克隆
表达
Dimocarpus longan
laccase
pericarp browning
gene cloning
expression
