围绝经期女性血清性激素水平与Th1/Th2平衡、外周血CD28、CTLA-4、PD-1、PD-L1表达的相关性

Correlation of serum sex hormone levels with Th1/Th2 balance as well as peripheral CD28,CTLA-4,PD-1 and PD-L1 expression in perimenopausal women

目的:研究围绝经期女性血清性激素水平与Th1/Th2平衡、外周血CD28、CTLA-4、PD-1、PDL1表达的相关性。方法:选择在本院妇科就诊的围绝经期女性和绝经女性作为围绝经期组和绝经组,同期体检的健康女性作为对照组。采集血清并测定Th1、Th2细胞因子及内皮损伤标志分子的含量,采集外周血并测定Th1、Th2细胞含量及CD28、CTLA-4、PD-1、PD-L1的mRNA表达量。结果:围绝经期组和绝经组女性外周血中Th1细胞的绝对值以及血清中IFN-γ、IL-2、ET-1的含量以及外周血单个核细胞中CD28、CTLA-4、PD-1、PD-L1的mRNA表达量显著高于对照组,外周血中Th2细胞的绝对值以及血清中IL-4、IL-10、NO的含量显著低于对照组;绝经组女性外周血中Th1细胞的绝对值以及血清中IFN-γ、IL-2、ET-1的含量以及外周血单个核细胞中CD28、CTLA-4、PD-1、PD-L1的mRNA表达量显著高于围绝经期组,外周血中Th2细胞的绝对值以及血清中IL-4、IL-10、NO的含量显著低于围绝经期组;外周血中Th1、Th2细胞的含量与ET-1的含量及CD28、CTLA-4、PD-1、PD-L1的mRNA表达量具有相关性。结论:围绝经期女性雌激素水平的降低会影响Th1/Th2的平衡,进而造成内皮功能损伤。

Objective： To study the correlation of serum sex hormone levels with Th1/Th2 balance as well as peripheral CD28, CTLA-4, PD-1 and PD-L1 expression in perimenopausal women.Methods： Perimenopausal women and postmenopausal women who were treated in Gynecology Department of Shenzhen Longhua District Central Hospital between May 2013 and October 2016 were selected as perimenopausal group and postmenopausal group, and healthy women who received physical examination during the same period were selected as control group.Serum was collected to detect the levels of Th1 and Th2 cytokines as well as endothelial injury marker molecules, and peripheral blood was collected to detect the levels of Th1 and Th2 cells as well as the mRNA expression of CD28, CTLA-4, PD-1 and PD-L1.Results： The absolute value of Th1 cells in peripheral blood, IFN-γ, IL-2 and ET-1 levels in serum as well as CD28, CTLA-4, PD-1 and PD-L1 mRNA expression in peripheral blood mononuclear cells of perimenopausal and postmenopausal group were significantly higher than those of control group while the absolute value of Th2 cells in peripheral blood as well as IL-4, IL-10 and NO levels in serum were significantly lower than those of control group;the absolute value of Th1 cells in peripheral blood, IFN-γ, IL-2 and ET-1 levels in serum as well as CD28, CTLA-4, PD-1 and PD-L1 mRNA expression in peripheral blood mononuclear cells of postmenopausal group were significantly higher than those of perimenopausal group while the absolute value of Th2 cells in peripheral blood as well as IL-4, IL-10 and NO levels in serum were significantly lower than those of perimenopausal group;peripheral blood Th1 and Th2 cell levels were correlated with ET-1 levels as well as CD28, CTLA-4, PD-1 and PD-L1 mRNA expression.Conclusion： Low estrogen levels in perimenopausal women can affect the Th1/Th2 balance and then result in endothelial injury.