食管癌组织中Slug表达量与癌细胞上皮-间质转化及侵袭特性的相关性

Correlation of Slug expression in esophageal cancer tissue with the epithelial-mesenchymal transition and invasion of cancer cells

目的:探讨食管癌组织中Slug表达量与癌细胞上皮-间质转化及侵袭特性的相关性。方法:收集在本院接受根治性手术治疗的食管癌患者78例,取术中食管癌组织及癌旁正常组织标本。采用荧光定量PCR法测定标本组织中Slug基因及侵袭基因的mRNA表达量,采用western-blot法测定上皮-间质转化相关指标的蛋白表达量,采用Pearson检验法评估食管癌组织中Slug表达量与癌细胞上皮-间质转化、侵袭活性的相关关系。结果:食管癌组织中Slug mRNA的表达量显著高于癌旁组织(P<0.05)。食管癌组织中上皮-间质转化标志物E-cadherin的蛋白表达量低于癌旁组织,Vimentin、N-cadherin的蛋白表达量高于癌旁组织,促侵袭基因Ezrin、FAT10、Survivin、MCP-1、RhoA的mRNA表达量均高于癌旁组织,侵袭抑制基因DEC1、PTEN、p53的mRNA表达量均低于癌旁组织(P均<0.05)。食管癌组织中Slug mRNA的表达量与上皮-间质转化标志物E-cadherin的蛋白表达量呈负相关,与Vimentin、N-cadherin的蛋白表达量呈正相关,与促侵袭基因Ezrin、FAT10、Survivin、MCP-1、RhoA的mRNA表达量呈正相关,与侵袭抑制基因DEC1、PTEN、p53的mRNA表达量呈负相关。结论:食管癌组织中Slug表达量高于癌旁组织,且Slug表达量与癌细胞上皮-间质转化及侵袭活性直接相关。

Objective： To study the correlation of Slug expression in esophageal cancer tissue with the epithelial-mesenchymal transition and invasion of cancer cells.Methods： A total of 78 patients with esophageal cancer who accepted radical operation in our hospital between June 2013 and June 2016 were selected, and the esophageal cancer tissue and normal tissue specimens adjacent to carcinoma were collected during operation.Fluorescence quantitative PCR method was used to determine the Slug gene and invasion gene mRNA expression in sample tissue, western-blot method was used to determine epithelial-mesenchymal transition-related index protein expression, and Pearson test was used to evaluate the correlation of Slug expression in esophageal cancer tissue with the epithelial-mesenchymal transition and invasion of cancer cells.Results： Slug mRNA expression in esophageal cancer tissue was significantly higher than that in tissue adjacent to carcinoma （P〈0.05）.Epithelial-mesenchymal transition marker E-cadherin protein expression in esophageal cancer tissue was lower than that in tissue adjacent to carcinoma while Vimentin and N-cadherin protein expression were higher than those in tissue adjacent to carcinoma, and pro-invasion genes Ezrin, FAT10, Survivin, MCP-1 and RhoA mRNA expression were higher than those in tissue adjacent to carcinoma while anti-invasion genes DEC1, PTEN and p53 mRNA expression were lower than those in tissue adjacent to carcinoma （all P〈0.05）.Slug mRNA expression in esophageal cancer tissue was negatively correlated with epithelial-mesenchymal transition marker E-cadherin protein expression, positively correlated with Vimentin and N-cadherin protein expression, positively correlated with pro-invasion genes Ezrin, FAT10, Survivin, MCP-1 and RhoA mRNA expression, and negatively correlated with anti-invasion genes DEC1, PTEN and p53 mRNA expression.Conclusions： Slug expression in esophageal cancer tissue is higher than that in tissue adjacent to carcinoma, and Slug expression is directly correlated with the epithelial-mesenchymal transition and invasion of cancer cells.