卵巢癌化疗前后肿瘤组织中PARP-1的表达及其与细胞上皮间质转化的相关性

Expression of PARP-1 and its correlation with epithelial mesenchymal transition in ovarian cancer before and after chemotherapy

目的:研究卵巢癌化疗前后肿瘤组织中PARP-1的表达及其与细胞上皮间质转化的相关性。方法:选择在本院接受紫杉醇联合卡铂方案(TC方案)化疗的晚期卵巢癌患者,化疗前和化疗后4个周期时分别采集肿瘤组织,测定PARP-1、上皮间质转化标志分子、血管新生分子的mRNA表达量。结果:化疗后,卵巢癌组织中PARP-1、Snail、ZEB1、N-cadherin、Vimentin、VEGF、VEGFR-1、VEGFR-2、Ang-2、Tie-2的mRNA表达量显著低于化疗前,E-cadherin的mRNA表达量显著高于化疗前;化疗后PARP-1低表达卵巢癌组织中Snail、ZEB1、N-cadherin、Vimentin、VEGF、VEGFR-1、VEGFR-2、Ang-2、Tie-2的mRNA表达量显著低于PARP-1高表达卵巢癌组织,E-cadherin的mRNA表达量显著高于PARP-1高表达卵巢癌组织。结论:TC方案化疗能够通过抑制卵巢癌病灶内PARP-1的表达来抑制上皮间质转化及血管新生过程。

Objective： To study the expression of PARP-1 in ovarian cancer tissues before and after chemotherapy and its correlation with epithelial mesenchymal transition.Methods： Advanced ovarian cancer patients received paclitaxel plus carboplatin （TC regimen） chemotherapy in Huangmei People＆#39;s Hospital during June 2013-August 2016 were collected.Before and 4 cycles after chemotherapy, tumor tissue were collected and mRNA expression of PARP-1 and epithelial mesenchymal transition markers, angiogenesis molecules were determined.Results： after chemotherapy, mRNA expression of PARP-1, Snail, ZEB1, N-cadherin, Vimentin, VEGF, VEGFR-1, VEGFR-2, Ang-2, Tie-2 in ovarian cancer were significantly lower than those before chemotherapy, mRNA expression of E-cadherin was significantly higher than that before chemotherapy;mRNA expression of PARP-1, Snail, ZEB1, N-cadherin, Vimentin, VEGF, VEGFR-1, VEGFR-2, Ang-2, Tie-2 in PARP-1 low expression ovarian cancer tissue were significantly lower than PARP-1 high expression ovarian cancer tissue, mRNA expression of E-cadherin was significantly higher than PARP-1 high expression ovarian cancer tissue.Conclusions： TC regimen can inhibit the process of epithelial mesenchymal transition and angiogenesis through inhibiting the expression of PARP-1 in ovarian cancer.