HS-SPME-GC×GC-TOFMS分析贵州茶叶的挥发性香气成分

Analysis on Volatile Aroma Components in Guizhou Tea by HS-SPME-GC×GC-TOFMS

为更全面的检测茶叶中的挥发性香气成分,采用顶空固相微萃取与全二维气相飞行时间质谱相结合(HS-SPME-GC×GC-TOFMS)的方法建立茶叶挥发性香气成分的分析方法,并通过质谱数据库匹配、内标相对定量、日内及日间相对标准偏差等参数进行方法验证。结果表明:该方法可对茶叶中77种挥发性香气成分进行定性定量分析,其质谱正、反匹配度均大于800,日内及日间相对标准偏差分别为11.3%和13.4%,方法的可靠性和稳定性较好。

In order to comprehensively detect volatile aroma components in tea,HS-SPME-GC×GCTOFMS was employed to analyze the volatile aroma components in tea,and the analytical performances were assessed in terms of method selectivity by mass spectrometry database match,internal standard relative quantitative method,intra-day and inter-day relative standard deviation（RSD）. Results：Qualitative and quantitative analysis could be conducted on 77 volatile aroma components in tea,and good qualitative accuracy was achieved in terms of match factors including match（SI）and reverse match（RSI）above 800 for all values and intra-day RSD11.3% deviation and inter-day RSD13.4%.All above indicate that this method is reliable and stable.