Effect of Helichrysum plicatum DC. subsp. plicatum ethanol extract on gentamicin-induced nephrotoxicity in rats

蜡菊乙醇提取物对庆大霉素诱导的大鼠肾毒性的影响（英文）

The aim of this study was to evaluate the possible therapeutic or protective effects of Helichrysum plicatum DC.subsp.plicatum ethanol extract（HPE）against gentamicin-induced nephrotoxicity.Thirty-six Sprague Dawley male rats weighing between 200 and 250 g were used as live material.They were formed into six groups containing 6rats each and were allowed to adapt to laboratory conditions for 7 d.Group Ⅰ：control,5%DMSO intraperitoneal（i.p.）;Group Ⅱ：HPE 100 mg/（kg·d）i.p.;Group Ⅲ：HPE 200 mg/（kg·d）i.p.;Group Ⅳ：gentamicin as 80 mg/（kg·d）i.p.;Group Ⅴ：gentamicin as 80 mg/（kg·d）i.p.＋HPE 100 mg/（kg·d）i.p.;and Group Ⅵ：gentamicin as 80 mg/（kg·d）i.p.＋HPE 200 mg/（kg·d）i.p.for 8 d.Following treatment,serum,liver,and kidney tissues were used to assess blood urea nitrogen（BUN）,creatinine,enzymatic and non-enzymatic antioxidants,and lipid peroxidation.Gentamicin significantly increased serum BUN,creatinin,and liver and kidney levels of malondialdehyde（MDA）.It also decreased the activity of catalase（CAT）,glutathione peroxidase（GPx）,and superoxide dismutase（SOD）.Treatment with the HPE 100 mg/kg reversed gentamicin-induced alterations as evidenced by decreased serum BUN and creatinin,liver and kidney oxidant marker,and tubular necrosis as well as by an increase in antioxidant enzymes.It was found that HPE 200 mg/kg significantly increased liver and kidney tissue MDA levels in nephrotoxicity in rats.As a result,these findings support the proposition that HPE in 100 mg/kg dose demonstrates in the kidney and liver as free radicals and scavenger to prevent the toxic effects of gentamicin in both the biochemical and histopathology parameters.

目的:评估蜡菊乙醇提取物(HPE)对庆大霉素诱导的肾毒性的治疗或保护作用。方法:将36只体重200~250 g的Sprague Dawley雄性大鼠分成6组,每组6只,适应实验室条件7 d。每组处理方式不同,包括:组Ⅰ,对照组,5%DMSO;组Ⅱ,HPE 100 mg/(kg·d);组Ⅲ,HPE200 mg/(kg·d);组Ⅳ,庆大霉素80 mg/(kg·d);组Ⅴ,庆大霉素80 mg/(kg·d)+HPE 100 mg/(kg·d);组Ⅵ,庆大霉素80 mg/(kg·d)+HPE 200 mg/(kg·d)。腹腔注射8 d后,取血清、肝和肾组织用于评估血液尿素氮(BUN)、肌酐、酶和非酶抗氧化剂和脂质过氧化。结论:庆大霉素能显著提升血清BUN、肌酐和肝肾阳性以及丙二醛(MDA)水平,同时降低过氧化氢酶(CAT),谷胱甘肽过氧化物酶(GPx)和超氧化物歧化酶(SOD)的活性。用100 mg/kg HPE的治疗能逆转庆大霉素诱导的改变。因此,100 mg/kg HPE在肾脏和肝脏中可作为自由基和清除剂,具有缓解庆大霉素在生物化学和组织病理学上毒性的作用。