分化型甲状腺癌组织中HSP70、E-cadherin、CyclinD1的表达变化与临床病理特征的关系

Expression of HSP70,E-cadherin and CyclinD1 in Differentiated Thyroid Carcinoma and Their Relationship with Clinicopathological Features

目的研究分化型甲状腺癌组织中热休克蛋白70(HSP70)、上皮钙粘蛋白(E-cadherin)、细胞增殖周期调控蛋白D1(Cyclin D1)的表达,探讨其与分化型甲状腺癌病理特征的关系。方法收集我院2013年1月~2016年5月存档的分化型甲状腺癌手术标本50例(乳头状瘤29例、滤泡状癌21例),甲状腺腺瘤18例,瘤旁正常甲状腺组织15例。采用免疫组化法检测组织标本中HSP70、E-cadherin、Cyclin D1的表达,并结合临床病理资料进行分析。结果分化型甲状腺癌组织中HSP70、Cyclin D1的阳性表达率显著高于甲状腺良性肿瘤组织及正常甲状腺组织(P<0.05),而E-cadherin的阳性表达率显著低于其它两组(P<0.05)。HSP70、Cyclin D1与分化型甲状腺癌的临床分期、淋巴结转移及包膜浸润有关(P<0.05),E-cadherin与分化型甲状腺癌的淋巴结转移及包膜浸润有关(P<0.05)。结论 HSP70、Cyclin D1在分化型甲状腺癌组织中呈高表达,E-cadherin在分化型甲状腺癌组织中呈低表达,且均与分化型甲状腺癌的发生、发展及转移密切相关。

Objective To investigate the expression of heat shock protein 70 （HSP70）, E-cadherin and CyclinD 1 in differentiated thyroid carcinoma and their relationship with pathologic features of differentiated thyroid carcinoma. Methods 50 cases of differentiated thyroid carcinoma （29 cases of papillary carcinoma, 21 cases of follicular carcinoma）, 18 cases of thyroid adenomas and 15 cases of normal thyroid tissues around the tumor were collected between January 2013 and May 2016 in our hospital. The expressions of HSP70, E-eadherin and CyclinD1 in the tissues were detected by immunohistoehemical method. Results The positive expression rate of HSP70 and CyclinD1 in dif- ferentiated thyroid carcinoma was significantly higher than that in benign thyroid tissue and normal thyroid tissue （P〈 0.05）, and the positive expression rate of E-cadhefin in differentiated thyroid carcinoma was significantly lower than that in the other two groups （P 〈 0.05）. The ex- pression of HSP70 and CyelinD1 in differentiated thyroid carcinoma were significantly correlated with clinical stage, lymph node metastasis and infiltration （P〈 0.05）, and E-eadherin was closely related with lymph node metastasis and infiltration （P〈 0.05）. Conclusion The high expression of HSP70 and CyclinD 1 in differentiated thyroid carcinoma and decreased expression of E-cadherin are closely related to the oc- currence, development and metastasis of differentiated thyroid carcinoma.