富血小板纤维蛋白在治疗糖尿病兔皮肤溃疡中的实验研究

Experimental study of platelet-rich fibrin in the treatment of diabetic rabbit with skin ulcers

目的观察富血小板纤维蛋白(PRF)对糖尿病动物模型皮肤创面的治疗作用及疗效。方法将20只大白兔随机分为空白对照组(4只)、糖尿病模型组(4只)、重组人表皮生长因子凝胶(rhEGF)组(4只),PRF组(8只),将糖尿病模型组、rhEGF组、PRF组建立糖尿病动物创面模型,4组动物创面分别予以1%碘伏清创、1%碘伏清创、rhEGF外敷、PRF外敷。观察各组创面面积及容积变化情况,标本予以HE染色观察创面组织生长情况,免疫组化标记血管内皮生长因子(VEGF)蛋白,计算VEGF平均光密度。结果干预后3、7 d显示,rhEGF组、PRF组创面面积和容积缩小的速度高于空白对照组及糖尿病模型组(P<0.05)。第2、3周末大体病理观察rhEGF组、PRF组创面组织表皮覆盖完全,真皮结构致密,皮肤结构基本恢复正常,有大量毛细血管存在。第2、3周末标本VEGF平均光密度蛋白含量,rhEGF组、PRF组显著高于空白对照组、糖尿病模型组(P<0.05)。结论 PRF可显著促进糖尿病兔皮肤创面的愈合。

Objective To ic animal model. Methods observe the therapeutic effect of platelet rich fibrin （PRF） on the skin wound of a diabet- 20 white rabbits were randomly divided into blank control group （n = 4）, diabetic model group （ n = 4 ）, recombinant human epidermal growth factor gel （rhEGF） group （ n = 4 ） , and PRF group （ n = 8 ）. Diabetic model group, rhEGF group and PRF group were established into a diabetic animal model. The wounds of the four groups were respectively handled by 1% iodine wound debridement, 1% iodophor debridement, rhEGF application, and PRF application. The changes of the wound area and volume were observed in each group. The morphology of the wound was observed by HE staining. The vascular endothelial growth factor（VEGF） protein was detected by immunohistochemical staining, and the mean optical density （MOD） of VEGF was calculated. Results The wound area and volume reduction rate in rhEGF group and PRF group were higher than that in blank control group and diabetic model group at 3 and 7 days after intervention （ P 〈 0. 05 ）. At 2 and 3 weeks, through gross pathological observation, the wound surface of rhEGF group and PRF group were covered with a complete skin covering, the dermis was dense, the structure of the skin was（ basically-not a very professional word, choose another like essentially） normal, and there was a large number of capillaries. The VEGF protein and PRF group in the tissue was determined by immunohistochemical examination and was content of rhEGF group significantly higher than those in the blank control group and diabetic model group （P 〈 0. 05 ）. Conclusion PRF can significantly promote the healing of skin wounds in diabetic rabbits