碎裂QRS波在大鼠心肌梗死模型中的价值探讨

The value of fragmentation QRS wave in rat myocardial infarction model

建立大鼠心肌梗死模型,探讨碎裂QRS波在大鼠心肌梗死模型中的价值。40只大鼠分为假手术组和心梗组,两组大鼠均在相同条件下进行手术,心梗组开胸并结扎大鼠冠状动脉前降支建立心肌梗死模型,假手术组只穿线不接扎。记录结扎(或穿线)10 min和60 min及术后4周大鼠心电图并分析碎裂QRS波及Q波变化规律。术后4周留取心肌梗死区组织标本,进行HE染色。与假手术组相比,心梗组大鼠在10、60 min及术后4周出现碎裂QRS波次数明显增多(P<0.01)。HE染色显示,心梗组部分心肌细胞排列紊乱,出现空泡变性。通过建立大鼠心肌梗死模型显示,碎裂QRS波可作为急性心肌梗死和陈旧性心梗诊断及预测疾病严重程度的新指标。

To set up rat myocardial infarction model and to investigate significance of fragmented QRS wave in rat myocardial infarction model. 40 rats were divided into sham-operated group and myocardial infarction group, these two sets of rats received surgeries under the same conditions. Myocardial infarction models were induced by opening chest and anterior descending branch of coronary artery deligation for rats. Rats in sham-operated group were only threaded without deligation. Recorded the electrocardiogram（ECG） during 10, 60 min and 4 weeks after the deli- gation（ or threading） and analyzed the change rules of the fragmented QRS waves and Q waves. Tissue samples from myocardial infarction area were collected for HE staining 4 weeks after the surgery. The frequency of fragmen- ted QRS wave in myocardial infarction group was significantly increased during 10, 60 min and at 4 weeks after the operation compared with sham-operated group（P 〈 0. 01 ）. Myocardial cells of some models in myocardial infarction group arranged in disorder and showed vacuolation according to results of HE staining. By setting up rat myocardial infarction model, we can draw a conclusion that fragmented QRS wave can be used as a new indicator to diagnose acute myocardial infarction and old myocardial infarction predict the severity of the disease.