葛根素和丹参酮ⅡA对维甲酸诱导骨质疏松大鼠生殖系统的修复作用

The effect of puerarin and tanshinone ⅡA on the restorative function of reproductive system in retinoic acid-induced osteoporosis in rats

目的探讨和比较葛根素(puerarin,PUE)、丹参酮ⅡA(tanshinoneⅡA)两种植物雌激素对维甲酸诱导的雌性骨质疏松大鼠生殖系统的修复作用。方法 12周龄SPF级健康雌性SD大鼠50只,随机分为正常组、模型组、葛根素组[50 mg/(kg·d)]、丹参酮ⅡA组[200 mg/(kg·d)]、苯甲酸雌二醇阳性对照组[40μg/(kg·d)]。除正常组外,其余各组每日给予维甲酸80mg/(kg·d)灌胃,建模21 d。建模21 d后除模型组外,其余各组进行给药,给药70 d后,检测各组大鼠体重,生殖系统(子宫、阴道、卵巢)湿重,ELISA测定血清雌二醇(estradiol,E2)含量,采集子宫、阴道组织进行石蜡包埋、HE染色,观察子宫及阴道形态,并用病理图像分析系统进行组织形态计量学测定。结果通过生殖系统脏器指数计算、血清雌二醇检测以及子宫和阴道组织形态计量学分析显示,模型组脏器指数、血清E2含量较正常组显著降低(P<0.05),模型组大鼠子宫腔径、管径厚度、上皮厚度、肌层厚度、腺体数量较正常组显著减少(P<0.01),阴道出现不同程度萎缩,阴道上皮厚度较正常组显著降低(P<0.01);葛根素组上述指标较模型组显著增加(P<0.05);丹参酮ⅡA组上述指标较模型组显著增加(P<0.01);丹参酮组与葛根素组相比,丹参酮组子宫管径厚度,阴道上皮厚度,显著高于葛根素组(P<0.01)。结论葛根素和丹参酮ⅡA均对维甲酸诱导的骨质疏松大鼠生殖系统损伤具有修复作用,且丹参酮ⅡA对生殖系统的修复作用优于葛根素。

Objective To study the restorative function of the two phytoestrogens,puerarin（PUE） and tanshinone ⅡA,on the reproductive system in retinoic acid-induced osteoporosis in rats. Methods Fifty 12-week-old healthy SD female rats were randomly divided into Normal group,Model group,Puerarin group（50 mg/kg·d）,Tanshinone ⅡA group（200 mg/kg·d）,and Estradiol benzoate positive control group（40 μg/kg·d）. Except for the Normal group,the rats in the rest groups received daily retinoic acid 80 mg/kg·d for 21 days. After 21-day modeling,except for the model group,the rats in other groups received drug for70 days. The weight of rats and the wet weight of their reproductive system（uterus,vagina,ovary） were detected after administration of drugs. The serum estradiol（E2） levels were determined with ELISA method. The rat uterus and vagina were collected for histopathological and histomorphometry analysis. Results By histomorphometric analysis,reproductive organ index calculation,and the serum estradiol detection,the organ index and E2 level in rats of model group had significant reduction comparing the those in normal group（P〈0. 05）. The diameter of the uterus,thickness of the uterine epithelium,myometrium layer,number of the uterine gland,and vaginal epithelial thickness also reduced（P〈0. 01）. Compared to those in the model group,the above indexes in Puerarin group increased（P〈0. 05）. In addition,the above indexes in Tanshinone ⅡA group increased significantly. Comparing to those in Puerarin group,the thickness of the uterine epithelium,myometrium layers,number of the uterine gland,and vaginal epithelial thickness increased significantly in Tanshinone ⅡA group（P〈0. 01）. ConclusionBoth PUE and tanshinone ⅡA have the restorative function on damaged reproductive system in osteoporotic rats induced by retinoic acid,and the repairing effect of tanshinone ⅡA is better than that of PUE on the reproductive system.