类弹性蛋白-蚕丝纤维复合材料的制备及性能研究

Fabrication and property study of elastin-like polypeptide and silk complex scaffold

采用热交联、京尼平交联、原花青素交联和戊二醛/聚乙二醇交联4种方法制备类弹性蛋白-蚕丝纤维多孔支架材料。利用扫描电镜、傅立叶变换红外光谱和力学性能测试研究了交联类弹性蛋白-蚕丝纤维多孔材料的内部形态和结构。通过细胞毒性实验分析了不同方法交联类弹性蛋白-蚕丝纤维多孔材料的细胞相容性。结果表明,热交联和京尼平交联能制备出的类弹性蛋白-蚕丝纤维多孔支架材料具有较高和较稳定的力学性能,且具有良好的细胞相容性。

Silk microfibers and Elastin-like polypeptide（ELP）were treated with four methods,（1）Dehydrothermal treatment（DHT）;（2）mixing with genipin;（3）mixing with proanthocyanidin（PA）;（4）mixing with PEG and glutaraldehyde（GA）.The resulting materials were characterized by scanning electron microscope（SEM）,Fourier transform infrared spectroscopy（FT-IR）and mechanical properties.The fiber reinforcement（SilkELP-DHT,Silk-ELP-Genipin and Silk-ELP-PEG-GA）resulted in the development of more mechanically robust constructs compared to silk alone.Meanwhile,the Silk-ELP-DHT and Silk-ELP-Genipin composite scaffolds show higher and more stable mechanical properties and better cytocompatibility.