赤胫散中总鞣质含量测定及提取工艺优化

Determination of Total Tannin in Polygonum runcinatum and Its Optimization of Extraction Process

目的:优化赤胫散中鞣质的提取工艺,建立赤胫散中总鞣质含量测定的方法。方法:采用磷钼钨酸-干酪素紫外分光光度法测定赤胫散中总鞣质含量,以提取物中总鞣质含量为评价指标,以碳酸钠浓度、提取时间和提取溶剂作为考察因素,采用正交设计法筛选赤胫散的提取工艺条件。结果:赤胫散中鞣质的线性范围为1~10 mg/L,相关系数为0.999 5,回收率(n=6)为99.06%(RSD=1.04)。结论:本方法简单、准确,可用于赤胫散中总鞣质含量的测定。

Objective： To optimize extraction process of total tannin in Polygonum runcinatum,and to establish stable determination method of total tannin content in Polygonum runcinatum. Method：Content of total tannin was detected by phosphomolybdic acid-casein UV spectrophotometry method with the content of total tannin as evaluation index,the sodium carbonate concentration,extraction（ ultrasound） time and extraction solvent as investigation factors,extraction process was optimized by orthogonal design method. Result： The linear range of tannin in Polygonum runcinatum was 1 ～ 10mg/L,the correlation coefficient was 0. 999 5,and the recovery rate was 99. 06%（ n = 6,RSD =1. 04）. Conclusion： The method is simple and accurate,and can be used to determine tannin content in Polygonum runcinatum.