深圳市南山区腹泻儿童腺病毒检测结果及其基因分型的研究

Study on adenovirus detection and its genotyping among children with diarrhea in Nanshan District in Shenzhen City

目的深入了解深圳市南山区腹泻病患儿腺病毒感染的发生率、临床特征、流行特点,以及主要致病亚型。方法以来深圳市南山区西丽人民医院就诊的深圳市南山区5岁以下腹泻病患儿的粪便标本为研究对象,通过多聚酶链反应(PCR)方法进行腺病毒核酸扩增;并将PCR阳性扩增产物进行克隆、测序,进行腺病毒基因分型的研究。结果 516例5岁以下腹泻病患儿共检出腺病毒感染患者57例,检出率为11.05%,其高发季节为3月、4月和8月;其中新生儿腹泻病患儿腺病毒的阳性感染者最低,各个年龄段之间腺病毒感染情况差异无统计学意义(χ~2=2.030,P=0.730)。57例腺病毒的阳性患者基因测序发现有30例腹泻病患儿感染F组Ad40和Ad41病毒(52.63%)。结论在深圳市南山区F组Ad40和Ad41病毒感染引起的腹泻发生率较高,相关部门应该加大对F组腺病毒感染腹泻的研究与预防。

Objective To investigate the incidence, clinical characteristics, epidemic characteristics and the main pathogenic subtypes of adenovirus infection among children with diarrhea in Nanshan District in Shenzhen City. Methods Stool specimens from children with diarrhea under 5 years old treated in our hospital from Nanshan District in Shenzhen City were selected as the research object, the adenovirus nucleic acid was amplified by polymerase chain reaction （PCR） method, and PCR positive amplification products were cloned and sequenced and the adenovirus genotyping was also studied. Results Among the 516 cases of children with diarrhea under the age of 5, 57 were detected with adenovirus infection, and the detection rate was 11.05%. The high-occurrence season was in March, April and August. The positive infection rate was the lowest in children with neonatal diarrhea and the difference of adenovirus infection among different ages was not statisticallyslgmficant（χ 2 = 2. 030, P = 0. 730） . 57 cases with positive adenovirus were conducted gene sequencing, and it was found that 30 children with diarrhea were infected with group F Ad40 and Ad41 virus （52. 63% ）. Conclusion The incidence of diarrhea caused by Group F Ad40 and Ad41 virus infection is high in Nanshan District of Shenzhen city, relevant departments should increase the research and prevention of adenovirus infection diarrhea of group F.