OIP5基因在肾癌中的表达及作用

Expression and function of OIP5 gene in renal cancer

目的:探讨OIP5基因与肾癌发生发展的相关性,及下调OIP5基因对肾癌细胞增值活力、侵袭力及成瘤能力的影响。方法:利用RT-PCR及Real-time PCR在4对肾癌组织及癌旁正常组织中检测OIP5的表达情况,利用Real-time PCR在肾正常细胞系HK-2及肾癌细胞系786-O中检测OIP5基因的表达情况。转染shRNA下调OIP5基因后,检测肾癌细胞的增殖活力、侵袭能力及成瘤能力等。进一步利用免疫组化的方法在裸鼠成瘤的组织中检测OIP5蛋白的表达。结果:我们发现OIP5基因在肾癌组织及肾癌细胞系中都明显的高表达。降低OIP5基因的表达后,肾癌细胞的增殖能力、侵袭能力及成瘤能力都明显的减弱了。结论:OIP5基因与肾癌的发生发展有关,OIP5可能是肾癌基因治疗的一个新靶点。

Objective：To investigate the relationship between OIP5 gene and the renal cancer risk,and the effect of down regulation of OIP5 gene expression on proliferation,invasion and tumorigenic ability of 786-O cell.Method：We used RT-PCR and real-time PCR to detect the OIP5 expression in four pairs of renal cell carcinoma tissues and adjacent normal tissues.At the same time,we detected OIP5 expression in HK-2and 786-O cell lines.After down regulation of OIP5 gene through transfecting shRNA,we detected proliferation,invasion and tumorigenic ability of 786-O cell.Result：We found OIP5 gene was obviously high expressed in renal cell carcinoma tissues and cell line.After reducing OIP5 gene expression,renal cancer cell proliferation,invasion and tumorigenic ability were significantly attenuate.Conclusion：OIP5gene is associated with the risk of renal cancer,which may be a new target of renal cancer gene therapy.