发光二极管630nm红光和460nm蓝光照射对日本大耳白兔皮肤创面愈合的影响

Effects of 630nm Red and 460nm Blue Light Emitting Diode Irradiation on Healing of the Skin Wound in Japanese Big-ear White Rabbit

目的观察发光二极管(LED)630 nm红光和460 nm蓝光照射对日本大耳白兔皮肤创面愈合的影响。方法采用日本大耳白兔8只,建立兔背部皮肤创伤模型,每只兔子3个伤口,分别给予红、蓝光距离15 cm垂直照射(15 min/次)和自然愈合处理。照射至创伤后第21天,观察各组创面愈合数目、愈合面积,计算创伤愈合面积百分比,比较两种光源的治疗作用;HE染色观察新生组织结构;Masson染色观察胶原纤维增生情况;免疫组织化学分析新生皮肤纤维细胞生长因子(FGF)、表皮生长因子(EGF)、内皮细胞生长因子(CD31)、增殖细胞核抗原(Ki-67)及炎性细胞因子(CD68)的表达情况。结果红光组、蓝光组和对照组的愈合率分别为50.0%(4/8)、25.0%(2/8)和12.5%(1/8)。自造模后第12天起,红光组伤口的愈合面积百分比均明显高于蓝光组及对照组(P<0.05或P<0.01)。建模后第21天,红光组新生皮肤厚度为(2.95±0.34)mm,明显高于对照组的(2.52±0.42)mm(F=3.182,P=0.016)。胶原纤维平均光密度为0.15±0.03,明显高于蓝光组的0.09±0.01(F=7.316,P=0.012)和对照组的0.07±0.01(F=7.316,P=0.003)。免疫组织化学检测结果显示,红光组的EGF、FGF、CD31抗原、Ki-67表达较蓝光组和对照组明显增多,CD68较蓝光组和对照组明显减少(P<0.05或P<0.01)。结论 LED红光照射可促进日本大耳白兔皮肤创面愈合,其可能是通过引起皮肤表皮细胞和血管内皮细胞生长及纤维组织增生来促成的。

Objective To observe the effects of 630 nm red light and 460 nm blue light emitting diode irradiation on the healing of skin wounds in Japanese big-ear white rabbits. Methods The skin wound model was established with 8 Japanese big-ear white rabbits. Three parts of vulnus in each rabbit were used： two parts of vulnus were irradiated vertically by red and blue LED light,respectively（15 min/time）,and the distance between lights and wounds was 15 cm; the 3rdpart of the wound was used as a control. On the 21 stday of the wounds exposure to light,the number of healing wounds and the percentage of healing area were recorded and the treatment effect of these two light sources was compared. HE staining was used to analyze the newborn tissue structure. Masson staining was used to observe the proliferation of skin collagen fibers. Immuohistochemical staining was used to analyze fibroblast growth factor（FGF）,epidermal growth factor（EGF）,endothelial growth factor（CD31）,proliferating cell nuclear antigen（Ki-67）,and inflammatory cytokines（CD68） infiltration in the skin. Results The healing rate in the red light,blue light,and control groups was 50. 0%（4/8）,25. 0%（2/8）,and 12. 5%（1/8）,respectively. Since the 12 thday after modeling,the healing area percentage in the red light group was significantly higher than those in the blue light and control groups（P〈0. 05,P〈0. 01）. On the 21 stday after modeling,the skin thickness of the red light group was（2. 95 ± 0. 34） mm,which was significantly higher than that in control group [（2. 52 ± 0. 42） mm; F = 3. 182,P = 0. 016） ]. The average optical density of collagen fibers was 0. 15 ± 0. 03 in red light group,which was significantly higher than that of the blue light group（0. 09 ± 0. 01; F = 7. 316,P = 0. 012） and control（0. 07 ± 0. 01; F = 7. 316,P = 0. 003）. The results of immunohistochemistry showed the expression levels of EGF,FGF,CD31 antigen,and Ki-67 in the red light group were significantly higher than those in the blue light and control groups,whereas the CD68 expression was significantly lower（P〈0. 05 or P〈0. 01）. Conclusion LED red light irradiation can promote the healing of skin wounds in Japanese big-ear white rabbits,which may be achieved by the effect of red light irradiation in stimulating the proliferation of skin epidermal cells,vascular endothelial cells,and fiberous tissue.