靶向融合防龋DNA疫苗研究(Ⅱ)—编码人Ig基因的质粒pJIg的构建

Study of Targeted Fusion Anticaries DNA Vaccine (Ⅱ)-Construction of Plasmid pJIg Encoding Human Immunoglobulinγ1 Gene Fragment.

目的 :构建编码人免疫球蛋白Igγ1铰链区和恒定区基因的质粒pJIg ,为进一步研制靶向融合防龋DNA疫苗做准备。方法 :从人外周淋巴细胞中获得细胞总RNA并合成cDNA第一链 ,利用半巢式PCR扩增Igγ1恒定区序列 ,进行T -A克隆 ,构建 pJIg质粒 ,酶切电泳 ,并测序鉴定。 结果 :RT -PCR获得了目的基因 ,质粒 pJIg携带有Igγ1目的片段基因。 结论 :成功构建编码人免疫球蛋白Igγ1恒定区基因的质粒 pJIg。

Objective:To construct the plasmid pJIg encoding the Fc region of human Igγ1 gene for the development of targeted fusion anti-caries DNA vaccine.Methods:The Fc region of human Igγ1 gene were amplified by RT-PCR from human peripheral lymphocyte.After that, the gene was cloned into pUCm-T vector to obtain the plasmid pJIg and then detected by electrophoresis and sequencing the gene.Results:Plasmid pJIg carried the Fc region of human Igγ1 gene.Conclusions:The recombinant plasmid pJIg carrying the Igγ1 Fc fragment was constructed successfully.