摘要
为探明试管薯诱导期温度对试管薯结薯率的影响效果,以马铃薯品种‘宁薯14号’为试验材料,在试管薯诱导期,研究了温度(13±1)℃、(15±1)℃、(17±1)℃、(19±1)℃和(21±1)℃对‘宁薯14号’结薯率的影响。结果表明,试管薯诱导期低温处理有利于促进试管薯结薯形成、缩短试管薯结薯时间,提高试管薯结薯率和单薯重。在全黑暗条件下,‘宁薯14号’在MS固体基本培养基+白糖(100 g/L)+6-BA(0.25 mg/L)+CCC(0.5 ml/L)培养基上,诱导期温度(17±1)℃,试管薯结薯效果最好,结薯率为100.0%,大中薯率为83.78%,单薯重57.0 mg。
The effects of temperature(13 ± 1)℃,(15 ± 1)℃,(17 ± 1)℃,(19 ± 1)℃ and(21 ± 1)℃ on microtuberization rate were studied using potato variety 'Ningshu 14' in order to investigate temperature effects on microtuberization in microtuber induction period. Low temperature treatment was favorable to mirotuberization, shortened the time to microtuberization, and increased microtuberization rate and weight per microtuber. The best results were achieved when single-node cuttings were cultured on MS solid medium + sugar(100 g/L) + 6-BA(0.25 mg/L) + CCC(0.5 ml/L) at(17 ± 1)℃ in full darkness, with microtuberization rate of 100.0%, large-and miedum-sized microtuber rate of 83.78% and single microtuber weight of 57.0 mg.
出处
《中国马铃薯》
2017年第3期134-137,共4页
Chinese Potato Journal
基金
宁夏农林科学院科技创新先导项目(NKYJ-16-13
NKYZ-16-0101)
宁夏自然科学基金项目(NZ14269)
宁夏农业育种专项(2014NYYZ01)
关键词
宁薯14号
试管薯诱导
温度
结薯率
Ningshu 14
microtuber induction
temperature
tuberization rate
