不同时间点肺爆震伤肺组织中TNF-α和NF-κB表达的变化

Expression changes of TNF-α and NF-κB in pulmonary blast injury rats at different time points

目的 建立大鼠爆震伤肺损伤模型,探讨不同时间点大鼠爆震伤肺损伤肺组织中肿瘤坏死因子-α（TNF-α）和转录因子-κB（NF-κB）表达的变化.方法 将36只SD大鼠随机分成6组：空白对照组,3、6、12、24、48 h爆震伤模型组,每组6只,实验结束后进行动物生命体征的观察,然后进行解剖留取大鼠肺脏组织标本,分别进行HE病理染色,应用荧光定量PCR仪和Western-blot方法进行不同时间点动物模型TNF-α和NF-κB分子和蛋白水平检测.结果大鼠肺脏HE染色结果显示,爆震伤导致小鼠肺组织产生大量炎症反应,肺泡壁增厚等症状.荧光定量PCR结果显示,TNF-α和NF-κB表达均显著升高,结果具有统计学意义.Western-blot结果显示,TNF-α从爆震伤后逐渐上升,并在12 h达高峰（3.76±0.17）,随后逐渐下降;NF-κB的表达随着时间延长逐渐升高,结果具有统计学意义.结论 爆震伤后肺脏中产生大量的炎症因子,且TNF-α和NF-κB基因和蛋白表达均上升,从而导致肺组织损伤.

Objective To establish a model of pulmonary blast injury in rats, to explore the expression changes in TNF-α and NF-κB at different time points of pulmonary blast injury.Methods Thirty-six SD rats were randomly divided into 6 groups： control group, 3, 6, 12, 24, and 48 h blast injury model group, with six rats in each group.At the end of the experimental time points, the animals were observed for signs of life and were sampled the lung tissue for pathological HE staining, fluorescence quantitative PCR and Western-blot to detect the expression level of TNF-α and NF-κB.Results HE staining showed that rat lung blast injury led to a large amount of lung tissue inflammation, alveolar wall thickening and other symptoms.Quantitative PCR showed that TNF-α and NF-κB mRNA expression in blast injury rats were significantly higher;Western-blot showed that, TNF-α increased gradually after the blast injury, and reached a peak at 12 h（3.76±0.17）, and then gradually decreased;expression of NF-κB expression with the extension of time gradually increased, the results were statistically significant.Conclusion Blast injury in lungs produces large amounts of inflammatory cytokines, and TNF-α and NF-κB mRNA and protein expression is increased, resulting in injury of lung tissue.