基于高通量测序技术分析使它隆对玉米土壤细菌多样性的影响

Effects of starane on maize soil bacterial diversity analyzed by high-throughput sequencing technology

【目的】研究使它隆对玉米土壤细菌多样性的影响。【方法】利用Illumina Miseq高通量测序技术,分别测定了玉米土壤细菌的16S rRNA基因的V4-V5可变区序列,进而对不同时期的不喷施除草剂和喷施除草剂的玉米土壤中细菌群落组成和多样性进行分析。【结果】研究共获得260940条有效序列,167191条优质序列,12656个OTUs。多样性分析结果表明,使它隆处理10 d后的土壤细菌多样性和丰度降低;使它隆处理60 d后的土壤细菌多样性和丰度提高。对土壤细菌群落组成分析发现,5个土壤样品中的优势菌门均为酸酐菌门、变形菌门、放线菌门、绿弯菌门和芽单胞菌门。使它隆处理10 d后的样品酸杆菌门的比例增加,放线菌门和绿弯菌门的比例降低;使它隆处理60 d后样品变形菌门的比例降低,绿弯菌门的比例明显增加。【结论】使它隆对玉米土壤细菌多样性产生一定影响,其影响随施药时间而异。

[Objective] To study the effects of starane on maize soil bacterial diversity. [Methods] We analyzed composition and structure of bacterial communities in maize soils from the control and spraying herbicide of different periods by Illumina MiSeq high-throughput sequencing of the V4-V5 region of the 16S rRNA gene. [Results] We received a total of 260940 effective sequences, 167191 high-quality sequences and 12656 OTUs. The soil bacterial diversity and abundance decreased after 10 days of starane treatment, whereas the soil bacterial diversity and abundance increased after 60 days of starane treatment. Bacterial community composition analysis shows that the dominant phyla in five soil samples were Acidobacteria, Proteobacteria, Actinobacteria, Chloroflexi and Gemmatimonadete. After 10 days of starane treatment, the ratio of Acidobacteria increased in herbicide treated soil, whereas the ratio of Actinobacteria and Chloroflexi decreased. After 60 days of starane treatment, Proteobacteria decreased, Chloroflexi increased obviously. [Conclusion] Starane affected maize soil bacterial diversity and its impact varied with the application time.