紫菜类菌孢素氨基酸抗皮肤光老化的研究

Effects of mycosporine-like amino acids from Porphyra haitanensis on skin photoaging

为探究从坛紫菜中提取的类菌孢素氨基酸(MAAs)化合物对紫外线诱导产生的小鼠皮肤光老化作用的保护机制,实验采用乙醇热水提取法,提取坛紫菜中的MAAs,对其化合物的基本成分组成进行鉴定;通过观测小鼠造模部位皮肤的损伤情况,结合皮肤病理切片来评价坛紫菜MAAs对光老化小鼠的保护作用,并通过测定小鼠组织中超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性及丙二醛(MDA)的含量和皮肤组织中羟脯氨酸(Hyp)的含量,分析MAAs化合物对光老化皮肤的保护机制。结果显示,从坛紫菜中提取的类菌孢素氨基酸类化合物主要组分为Porphyra-334和Shinorine;紫菜MAAs可有效改善因紫外线诱导而产生的皮肤光老化的发生;与模型组小鼠相比,外涂给药组小鼠皮肤组织中SOD、GSH-Px的活性及Hyp的含量显著性提高,MDA的含量显著降低;而灌胃给药组与空白对照组小鼠相比体内抗氧化酶活性作用水平变化不明显。研究表明,紫菜MAAs对紫外线诱导的皮肤光老化具有保护作用。

To investigate mechanism of mycosporine-like amino acid from Prophyra against ultraviolet irradiationinduced photoaging of skin and provide an experimental basis for clinical application of sunscreen cosmetics,MAAs derived from Prophyra haitanensis was extracted using hot water and the components of MAAs were separated and identified. The protective effects of MAAs on photoaging were observed by the degree injury of tissue appearance and pathological section. The protective mechanism of mycosporine-like amino acid from Prophyra against ultraviolet irradiation-induced photoaging of skin was measured by the activities of total superoxide dismutase（T-SOD）, glutathione peroxidase（GSH-Px） and the levels of malondialdehyde（MDA）and hydroxyproline（Hyp）. The results indicated that two major mycosporine-like amino acid components from Prophyra were separated： Porphyra-334 and shinorine. MAAs could prevent skin photoaging induced by ultraviolet irradiation. Compared with the control group, MAAs treatment group external inunctum exhibited significantly enhanced activity of T-SOD and GSH-Px, increased levels of Hyp, and reduced levels of MDA. But the groups of MAAs treatment external inunctum on antioxidant showed that it couldn＇t have significant action compared with MAAs group gastric lavage. The mycosporine-like amino acid from Prophyra could protect skin from ultraviolet irradiation-induced skin photoaging.