摘要
AIM： To analyze cytokines as biomarkers for evaluation of severity of glaucoma. METHODS： This was a prospective case-control study including 29 eyes with glaucoma. Besides, 28 eyes with senile cataract were used as control. Patients were classified into four groups： acute angle closure glaucoma （AACG）, chronic angle closure glaucoma （CACG）, primary open angle glaucoma （POAG） and senile cataract. Undiluted vitreous samples were collected, then vitreous concentrations of 9 types of cytokines were determined by cytometric bead assay system： y-interferon （IFNg）, interleukin （IL-10, IL-2, IL-4, IL-5, interferon-y-inducible protein （IP）-10, monocyte chemoattractant protein （MCP）-I, tumor necrosis factor （TNF） -Q, and vascular endothelial growth factor （VEGF）. We also recorded the intraocular pressure （lOP） of patients in each group and Pearson correlated analysis was performed to analysis the correlation between each type of cytokine with IOR RESULTS： Vitreous levels of IL-2, iL-5, MCP-1, TNF-a and IP-10 were significantly higher （P〈0.05） in AACG group. Patients with AACG, CACG and POAG have higher lOP than senile cataract, but we didn＇t find any significant correlation between lOP with any type of the cytokines. CONCLUSION： Inflammation and immune reaction have a strong link with the pathology of glaucoma especially AACG, Some cytokines may act as biomarkers to evaluate the severity of glaucoma, Anti-inflammatory treatments and controlling of lOP are necessary for the therapy of glaucoma.
AIM： To analyze cytokines as biomarkers for evaluation of severity of glaucoma. METHODS： This was a prospective case-control study including 29 eyes with glaucoma. Besides, 28 eyes with senile cataract were used as control. Patients were classified into four groups： acute angle closure glaucoma （AACG）, chronic angle closure glaucoma （CACG）, primary open angle glaucoma （POAG） and senile cataract. Undiluted vitreous samples were collected, then vitreous concentrations of 9 types of cytokines were determined by cytometric bead assay system： y-interferon （IFNg）, interleukin （IL-10, IL-2, IL-4, IL-5, interferon-y-inducible protein （IP）-10, monocyte chemoattractant protein （MCP）-I, tumor necrosis factor （TNF） -Q, and vascular endothelial growth factor （VEGF）. We also recorded the intraocular pressure （lOP） of patients in each group and Pearson correlated analysis was performed to analysis the correlation between each type of cytokine with IOR RESULTS： Vitreous levels of IL-2, iL-5, MCP-1, TNF-a and IP-10 were significantly higher （P〈0.05） in AACG group. Patients with AACG, CACG and POAG have higher lOP than senile cataract, but we didn＇t find any significant correlation between lOP with any type of the cytokines. CONCLUSION： Inflammation and immune reaction have a strong link with the pathology of glaucoma especially AACG, Some cytokines may act as biomarkers to evaluate the severity of glaucoma, Anti-inflammatory treatments and controlling of lOP are necessary for the therapy of glaucoma.
作者
Yao Tong
Ya-Li Zhou
Yan Zheng
Manas Biswal
Pei-Quan Zhao
Zhao-Yang Wang
Yao Tong;Ya-Li Zhou;Yan Zheng;Manas Biswal;Pei-Quan Zhao;Zhao-Yang Wang(Department of Ophthalmology,Shanghai Ninth People’s Hospital,Shanghai Jiaotong University School of Medicine;Department of Ophthalmology,Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine;Department of Ophthalmology,Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine,Chongming Branch;Department of Molecular Genetics,University of Florida)
基金
Supported by the National Natural Science Fundation of China(No.81371040)
Shanghai Pujiang Program(No.15PJD028)
Scientific Research Project of Shanghai Municipal Health Bureau(No.20124149)
