ALDH2基因和其他脂质代谢基因在麻风患者外周血中的表达

The Expressions of Gene Aldehyde Dehydrogenase 2 and Lipid Metabolism Genes in Leprosy

目的研究乙醛脱氢酶2(aldehyde dehydrogenase 2,ALDH2)基因和脂质代谢与麻风的相关性。方法入选27例麻风病例和18例健康对照,转录组测序的方法比较了麻风病例皮损和健康对照中22个脂质代谢相关基因mRNA表达水平的差异。q PCR的方法检测了18例麻风患者和26例健康对照的外周血中ALDH2基因mRNA的表达水平。酶联免疫吸附测定(ELISA)的方法检测27例麻风患者和18例健康对照血清中ALDH2的浓度。结果麻风病例组和对照组皮损中22种脂质代谢相关基因表达差异有统计学意义(P<0.05);麻风病例外周血中ALDH2的mRNA水平与健康对照差异无统计学意义(P=0.50);麻风病例血清中ALDH2浓度低于对照组,差异有统计学意义(P<0.01)。结论麻风病例的脂质代谢相关基因与健康对照相比存在显著差异。麻风病例血清中乙醛脱氢酶2浓度显著低于健康对照,ALDH2可能通过调节脂质代谢影响麻风分枝杆菌对宿主的感染和麻风的发病。

Objective To study the associations of Aldehyde dehydrogenase 2 gene and lipid metabolism with leprosy. Methods The expressions of lipid metabolism related genes in 27 leprosy patients and 18 healthy controls were compared by comparative RNA sequencing. The expression of gene ALDH2 in the peripheral blood of 11 patients and 10 controls were determined using quantitative PCR. And concentrations of ALDH2 in the serum of 27 patients and 18 controls were measured by enzyme-linked immunosorbent assay. Results The expressions of 22 selected genes involved in the lipid metabolism showed remarkable differences between patients and controls（P〈0.05）. The difference of ALDH2 mRNA levels in peripheral blood was not significant between leprosy patients and normal controls（P=0.50）. Concentration of ALDH2 in leprosypatients＇ serum was obviously lower than that in healthy controls（P〈0.01）. Conclusion Lipid metabolism related genes were remarkably differentally expressed between leprosy patients and healthy controls. The concentration of ALDH2 was significantly lower in leprosy patients than that in healthy controls. It is possibile that ALDH2 affected the onset and development of leprosy by regulating lipid metabolism.