沙眼衣原体荧光PCR法在阴道炎患者实验室检测中的效果评价

Evaluation of the Effect of Chlamydia Trachomatis Fluorescent PCR Method in the Detection in the Laboratory Test of Vaginitis Patients

目的评价沙眼衣原体(Chlamydia Trachomatis,CT)荧光PCR法在阴道炎患者实验室检测中的作用。方法选取闵行区中心医院妇科门诊760例有临床症状患者,采集其阴道分泌物进行白带常规检查、HE染色、细菌性阴道病(BV)检测,并运用荧光PCR法检测沙眼衣原体和解脲脲原体(Ureaplasma urealyticum,UU),并对CT进行相对定量分析。结果 CT阳性率14.87%,UU阳性率61.98%,显著高于真菌性阴道炎(7.24%)、滴虫性阴道炎(3.68%)及BV(10.39%),差异均有统计学意义(P<0.05)。而且CT在性活跃人群(<25岁)感染率最高。在排除UU、真菌感染、滴虫感染和BV后,妇科门诊患者根据白带常规和HE染色结果被分为阴道炎和非阴道炎两组,两组CT的感染率分别为14.29%和6.41%,差异具有统计学意义(P=0.040),CT拷贝对数值分别为5.88±0.99和4.79±1.17,两组具有统计学意义(P=0.025)。结论 CT是阴道炎重要病原菌之一,CT感染载量与阴道炎发生密切相关,应对妇科门诊及住院患者进行常规CT筛查。

Objective To evaluate the role of chlamydia trachomatis（CT）PCR fluorescence detection in patients with vaginitis.Methods Seven hundred and sixty samples of vaginitis patients with clinical symptomsin Minhang Hospital were selected,collection of vaginal secretions for routine vaginal examination, HE staining, bacterial vaginal disease（BV）detection, and the use of fluorescent PCR method for detection of chlamydia trachomatis and ureaplasma urealyticum.In addition, the relative quantitative analysis of CT.Results The positive rate of CT was 14.87% and the positive rate ofUU was 61.98% in patients with gynecologicalclinic,significantly higher than that of fungal vaginitis（7.24%）,trichomoniasis vaginitis（3.68%）and BV（10.39%）,（P〈0.05）.Moreover, CT in the sexually active population（〈25yearsold）infection rate is the highest.After the exclusion of the ureaplasmaurealyticum,fungal infection, trichomonas infection and BV. Gynecological outpatients according to the leucorrhea routine and HE staining results were divided into vaginitis group and no-infection vaginitis group, the vaginitis group of CT infection rate was 14.29% and the no-infection vaginitis group of CT infection rate was 6.41%, with the between two groups was statistically significant（P= 0.040）.The logarithm of CTin the vaginitis group and in the no-infection vaginitis groupwere 5.88±0.99 and 4.79±1.17 respectively with significant differences（P=0.025）.Conclusion CT is one of the important pathogenic bacteria causing gynecological vaginitis.The amount of CT infection was closely related to the occurrence of vaginitis. It is necessary to carry out routine CT screening for gynecological outpatients and inpatients.