肿瘤转移抑制基因-1在食管鳞癌组织及食管癌EC109细胞中的表达及意义

Expression and significance of TMSG-1 in esophageal squamous carcinoma and EC109 cells

目的探讨肿瘤转移抑制基因-1(TMSG-1)在食管鳞癌(ESCC)组织及食管癌EC109细胞中的表达及意义。方法采用SP免疫组织化学染色法检测136例ESCC及37例正常食管黏膜组织中TMSG-1蛋白的表达情况,分析TMSG-1与ESCC患者临床病理指标的关系;培养人食管癌EC109细胞并用常用化疗药物顺铂(CDDP)干预,同时设对照组,采用MTT法检测细胞增殖抑制率,半定量RT-PCR法检测细胞TMSG-1的表达情况。结果 TMSG-1在ESCC和正常食管黏膜的阳性率分别为52.2%(71/136)、94.6%(35/37),差异具有统计学意义(P<0.05)。TMSG-1的异常表达与ESCC组织学分级、患者的TNM分期、淋巴结转移情况相关。顺铂干预组EC109细胞的增殖抑制率较对照组显著增高,差异具有统计学意义(P<0.01)。RT-PCR结果显示,干预组EC109细胞TMSG-1的mRNA表达明显高于对照组(P<0.01)。结论 TMSG-1的异常表达与ESCC的发展及转移相关,可作为较为理想的肿瘤标志物辅助诊断并指导临床治疗。

Objective To study the expression and significance of tumor metastasis suppressor gene-1（TMSG- 1） in esophageal squamous cell carcinoma （ESCC） and EC109 cells. Methods Immunohistochemistry S-P method was used to examine the expression of TMSG-1 protein in 136 cases of ESCC and 37 cases of normal esophageal mucosa. We analyzed the relationship between TMSG-1 and clinicopathological data of ESCC patients. EC109 cells were treated with 3 μg/mL of cisplatin （CDDP） in vitro for 24 h （the intervention group） and the control group was set up at the same time. The proliferation-inhibitory capability was analyzed with MTT assay. RT-PCR was used to examine the expression of TMSG-1 in the intervention group and the control group. Results The positive rate of TMSG-1 in ESCC and normal esophageal mucosa was 52. 2% （71/136） and 94. 6% （35/37）, respectively. The expression of TMSG-1 in ESCC was significantly lower than that in normal esophageal mucosa （P〈0.05）. The expression of TMSG-1 was related to TNM stage, differentiation degree and lymph node metastasis （P〈 0.05）. After EC 109 cells were treated with CDDP for 24 h, the proliferation inhibition rate was increased significantly compared with the control group （P〈0.01）. RT-PCR results showed that the expression of TMSG-1 in the cells of the intervention group was significantly higher than that in the control group （P〈 0. 01）. Oonclusion The abnormal expression of TMSG-1 may play a role in the development and metastasis of ESCC. Examination of TMSG-1 may be useful for making diagnosis and guiding clinical therapy of ESCC.