温胆汤对帕金森病模型大鼠抑郁样行为及脑内单胺类神经递质的影响

Effects of Wendan decoction on depression-like behavior and cerebral monoamine neurotransmitters in a rat model of Parkinson's disease

目的观察温胆汤对帕金森病(Parkinson’s disease,PD)模型大鼠抑郁样行为的作用,并探讨其相关机制。方法以6-羟多巴胺损毁大鼠单侧内侧前脑束建立PD模型,用温胆汤灌胃治疗,采用蔗糖偏好和强迫游泳实验检测大鼠抑郁样行为的改变,高效液相色谱-电化学检测法检测相关脑区单胺类神经递质含量的变化。结果与假手术组相比,模型大鼠蔗糖消耗量显著减少,强迫游泳不动时间明显延长;内侧前额叶皮层内多巴胺、5-羟色胺和去甲肾上腺素含量显著减少,纹状体内多巴胺含量明显减少。经温胆汤灌胃治疗2周后,模型大鼠蔗糖消耗量显著增加,强迫游泳的不动时间显著缩短,内侧前额叶皮层中多巴胺、5-羟色胺和去甲肾上腺素含量明显增加。结论温胆汤可以改善PD伴发的抑郁样行为,对内侧前额叶皮层内单胺类神经递质的调节可能是温胆汤发挥疗效的机制之一。

Objective To observe the effects of Wendan decoction （WD） on depression-like behavior in a rat model of Parkinson＇s disease （PD） and explore the related mechanism. Methods Rodent model of PD was established by unilaterally lesioning medial forebrain bundle with 6-hydrodopamine. After intragastric administration with WD, the rats＇s behavior changes were detected by the open field test, sucrose preference test and forced swimming test; the contents of monoamine neurotransmitters in the rat brain were assessed by high-performance liquid chromatography with electrochemical detection. Results Compared with those of sham-operated rats, the horizontal and vertical activities of the PD model rats decreased significantly, and sucrose consumption decreased significantly, but immobility time during forced swimming was significantly prolonged. The contents of dopamine （DA）, 5-hydroxytryptamine （5-HT） and noradrenaline （NA） in the medial prefrontal cortex （mPFC） and DA in the striatum decreased significantly. After administration of WD for 2 weeks, the immobility time of the PD model rats was significantly decreased, sucrose consumption increased significantly; DA, 5-HT and NA levels in the mPFC increased significantly. Conclusion WD improves the depression-like behavior in PD model rats, and the mechanisms may involve the regulation of monoamine neurotransmitters in mPFC.