乙肝病毒相关肝细胞肝癌与IL-32表达相关性研究

Expression of interleukin-32 in hepatitis B virus related hepatocellular carcinoma

目的肝细胞肝癌(hepatocellular carcinoma,HCC)是最常见的恶性肿瘤之一,白细胞介素(interleukin,IL)-32是新发现的一种细胞因子,在HCC中的研究较少。本研究探讨其在乙肝病毒(heptitis B virus,HBV)相关HCC患者外周血、肝组织以及体外培养的HepG2、HepG2 2.15细胞株中表达水平的变化。方法选择2013-01-01-2016-06-30在南通市第三人民医院住院的HBV相关肝硬化(liver cirrhosis,LC)27例和HBV-HCC 29例作为研究对象,选择体检健康者17名作为对照组,采用酶联免疫吸附方法检测外周血IL-32。选择外科手术切除HBV-HCC、癌旁LC标本20份,采用免疫组织化学的方法检测肝组织中IL-32表达情况。对HepG2、HepG2 2.15细胞进行培养,72h后采集上清液,采用蛋白印迹法检测IL-32。结果 HBV-HCC患者外周血IL-32表达水平为(479.46±204.46)ng/L,高于HBV-LC患者(333.61±162.76)ng/L;HBV-LC患者高于健康对照组为(75.58±9.90)ng/L,P<0.005;HBV-HCC癌组织中IL-32表达水平高于癌旁LC组织,Z=2.725,P=0.006;HepG2细胞株与HepG2.2.15细胞株培养72h后,上清液中未能检测到IL-32。结论 IL-32在HBV相关HCC外周血、HCC癌组织中均处于过度表达状态,在HBV-HCC的发生发展过程中起到一定的作用。

OBJECTIVE Hepatocellular carcinoma（HCC）was one of the most common malignant tumors,interleukin（IL）-32 was a new cytokine,but few studies about it was found in HCC.The aim of this study was to explore the changing of IL-32 in peripheral blood and liver tissue with hepatitis B virus（HBV）related HCC and to detect the level of IL-32 in the cell lines of HepG2、HepG2 2.15 in vitro.METHODS In the third People’s hospital of Nantong from2013-01-01 to 2016-06-30,27 patients with HBV related liver cirrhosis（LC）,29 patients with HBV-HCC,17 healthy controls were enrolled in the study,enzyme linked immunosorbent assay（ELISA）was used to detect peripheral IL-32.Twenty groups of liver tissue with HBV-HCC or HBV-LC were used to test IL-32 with immunohistochemistry.Cell lines including human hematoma HepG2 and HepG2 2.15 were cultured for 72 hours,West blotting was used to detect IL-32 in supernatant.RESULTS Peripheral IL-32 in patients with HBV-HCC [（479.46±204.46）ng/L]was more than with HBV-LC [（333.61±162.76）ng/L].In patients with HBV-LC was more than with healthy controls [（75.58±9.90）ng/L],P〈0.005;The level of IL-32 in liver tissue with HBV-HCC was higher than that with LC（Z=2.725,P=0.006）.But IL-32 was not detected in supernatant in vitro when the cell lines of HepG2 and HepG2.2.15 were cultured for 72 hours.CONCLUSIONS IL-32 is over expression in peripheral blood and liver tissue with HBV-HCC.IL-32 might play a role in the promotion of HBV-HCC.