食管鳞癌组织中锌α2糖蛋白的表达及其临床意义

Expression of Zinc α2-glycoprotein in the Esophageal Squamous Cell Carcinoma

目的探讨食管鳞癌组织中ZAG蛋白的表达及其临床意义。方法采用免疫组化法检测85例食管鳞癌组织和对应的正常食管黏膜组织中ZAG蛋白的表达,并分析ZAG蛋白表达与食管鳞癌患者预后之间的关系。结果 ZAG蛋白表达主要定位于细胞质中,其在食管鳞癌组织中表达的阳性率为38.8%(33/85),显著低于正常食管黏膜组织的90.6%(77/85),差异有统计学意义(χ~2=49.867,P<0.001)。ZAG蛋白表达与食管鳞癌患者的组织学分级、TNM分期和淋巴结转移关系密切(P<0.05),但与患者的性别、年龄无关(P>0.05)。ZAG蛋白高表达的食管鳞癌患者生存时间显著长于ZAG低表达的食管鳞癌患者(P<0.05)。结论 ZAG在食管鳞癌发生、发展中发挥极其重要的作用,并可能与食管鳞癌患者的预后密切相关,因而可能成为食管鳞癌预后的分子标记。

Objective To study the expression and clinical significant of zinc α2-glycoprotein （ZAG） in human esophageal sqnamous cell carcinoma tissue. Methods Immunohistochemistry method was to detect the protein of ZAG in the 85 cases of esophageal squamous cell carcinoma and adjacent normal esophageal epithelial tissues. The relation of ZAG expression with survival time was analyzed. Results ZAG protein expression was localized in cytoplasm, and its expression in the esophageal squamous cell carcinoma tissues [ 38.8% （33/85）] was significantly lower than that in the normal esophageal epithelial tissues [ 90.6% （77/85） ], and there was statistical difference （x^2 = 49. 867 ,P 〈 0.001 ）. The expression of ZAG protein was correlated with histological differentiation, TNM staging and lymph node metastasis （ P 〈 0.05 ）, but was not associated with gender and age of the patients with esopha- geal squamons cell carcinoma. The patients with high ZAG level had longer survival time than those with low ZAG level （P 〈 0.05 ）. Conclusion ZAG plays a pivotal role in the occurrence and development of esophageal squamous cell carcinoma, and may be related to the prognosis of patients with esophageal squamous cell carcinoma closely. ZAG may be molecular marker for the pregnosis of the patients with esophageal squamous cell carcinoma.