摘要
目的 评价氢对老龄小鼠术后认知功能的影响。方法 老龄雄性昆明小鼠60只,12~14月龄,体重35~45 g。采用随机数字表法分为3组(n=20):假手术组(S组)、肝部分切除术组(PH组)和肝部分切除术+富氢液组(PH-H组)。于术前3 d、术中以及术后3 d,PH-H组腹腔注射富氢液10 ml/kg,S组和PH组腹腔注射等体积生理盐水,1次/d。术后7 d行空间探索实验,以评价认知功能。于术后3和7 d(空间探索实验结束后)时分别随机取10只小鼠,取血标本,采用ELISA法检测血清TNF-α、IL-1β和高迁移率蛋白B1(HMGB1)浓度,处死取脑组织,分离海马,采用Western blot法检测海马TNF-α、IL-1β、HMGB1表达水平。结果 与S组比较,PH组目标象限停留时间百分比、1 min穿越平台次数降低,血清TNF-α、IL-1β、HMGB1浓度升高,海马TNF-α、IL-1β、HMGB1表达上调(P〈0.05),PH-H组上述指标差异无统计学意义(P〉0.05);与PH组比较,PH-H组目标象限停留时间百分比、1 min穿越平台次数升高,血清TNF-α、IL-1β、HMGB1浓度降低,海马TNF-α、IL-1β、HMGB1表达下调(P〈0.05)。结论 氢可改善老龄小鼠术后认知功能。
Objective To evaluate the effect of hydrogen on the postoperative cognitive function in aged mice.Methods Sixty aged male Kunming mice, aged 12-14 months, weighing 35-45 g, were assigned into 3 groups(n=20 each)using a random number table: sham operation group(group Sham), partial hepatectomy group(group PH)and partial hepatectomy plus hydrogen-enriched saline group(group PH-H). On preoperative day 3, during operation and on postoperative day 3, hydrogen-enriched saline 10 ml/kg was intraperitoneally injected once a day in group PH-H, and the equal volume of normal saline was intraperitoneally injected once a day in S and PH groups. The spatial probe test was performed on postoperative day 7 to evaluate the cognitive function. Ten mice were selected on postoperative days 3 and 7(after the end of the spatial probe test), and blood samples were collected for determination of serum tumor necrosis factor-alpha(TNF-α), interleukin-1beta(IL-1β)and high-mobility group box 1 protein(HMGB1)concentrations by enzyme-linked immunosorbent assay.The animals were sacrificed after blood sampling, brains were removed and hippocampi were isolated for detection of the expression of TNF-α, IL-1β and HMGB1 by Western blot.Results Compared with group S, the percentage of time of staying at the target quadrant and the number of crossing the platform within 1 min were significantly decreased, the serum concentrations of TNF-α, IL-1β and HMGB1 were increased, and the expression of hippocampal TNF-α, IL-1β and HMGB1 was up-regulated in group PH(P〈0.05), and no significant change was found in the variables mentioned above in group PH-H(P〉0.05). Compared with group PH, the percentage of time of staying at the target quadrant and the number of crossing the platform within 1 min were significantly increased, the serum concentrations of TNF-α, IL-1β and HMGB1 were decreased, and the expression of hippocampal TNF-α, IL-1β and HMGB1 was down-regulated in group PH-H(P〈0.05).Conclusion Hydrogen can improve the postoperative cognitive function in aged mice.
出处
《中华麻醉学杂志》
CSCD
北大核心
2017年第5期581-584,共4页
Chinese Journal of Anesthesiology
关键词
氢
认知障碍
手术后并发症
老年人
Hydrogen
Postoperative complications
Cognition disorders
Aged