氢对老龄小鼠术后认知功能的影响

Effect of hydrogen on postoperative cognitive function in aged mice

目的 评价氢对老龄小鼠术后认知功能的影响。方法 老龄雄性昆明小鼠60只，12～14月龄，体重35～45 g。采用随机数字表法分为3组（n＝20）：假手术组（S组）、肝部分切除术组（PH组）和肝部分切除术＋富氢液组（PH-H组）。于术前3 d、术中以及术后3 d，PH-H组腹腔注射富氢液10 ml/kg，S组和PH组腹腔注射等体积生理盐水，1次/d。术后7 d行空间探索实验，以评价认知功能。于术后3和7 d（空间探索实验结束后）时分别随机取10只小鼠，取血标本，采用ELISA法检测血清TNF-α、IL-1β和高迁移率蛋白B1（HMGB1）浓度，处死取脑组织，分离海马，采用Western blot法检测海马TNF-α、IL-1β、HMGB1表达水平。结果 与S组比较，PH组目标象限停留时间百分比、1 min穿越平台次数降低，血清TNF-α、IL-1β、HMGB1浓度升高，海马TNF-α、IL-1β、HMGB1表达上调（P〈0.05），PH-H组上述指标差异无统计学意义（P〉0.05）；与PH组比较，PH-H组目标象限停留时间百分比、1 min穿越平台次数升高，血清TNF-α、IL-1β、HMGB1浓度降低，海马TNF-α、IL-1β、HMGB1表达下调（P〈0.05）。结论 氢可改善老龄小鼠术后认知功能。

Objective To evaluate the effect of hydrogen on the postoperative cognitive function in aged mice.Methods Sixty aged male Kunming mice, aged 12-14 months, weighing 35-45 g, were assigned into 3 groups（n=20 each）using a random number table： sham operation group（group Sham）, partial hepatectomy group（group PH）and partial hepatectomy plus hydrogen-enriched saline group（group PH-H）. On preoperative day 3, during operation and on postoperative day 3, hydrogen-enriched saline 10 ml/kg was intraperitoneally injected once a day in group PH-H, and the equal volume of normal saline was intraperitoneally injected once a day in S and PH groups. The spatial probe test was performed on postoperative day 7 to evaluate the cognitive function. Ten mice were selected on postoperative days 3 and 7（after the end of the spatial probe test）, and blood samples were collected for determination of serum tumor necrosis factor-alpha（TNF-α）, interleukin-1beta（IL-1β）and high-mobility group box 1 protein（HMGB1）concentrations by enzyme-linked immunosorbent assay.The animals were sacrificed after blood sampling, brains were removed and hippocampi were isolated for detection of the expression of TNF-α, IL-1β and HMGB1 by Western blot.Results Compared with group S, the percentage of time of staying at the target quadrant and the number of crossing the platform within 1 min were significantly decreased, the serum concentrations of TNF-α, IL-1β and HMGB1 were increased, and the expression of hippocampal TNF-α, IL-1β and HMGB1 was up-regulated in group PH（P〈0.05）, and no significant change was found in the variables mentioned above in group PH-H（P〉0.05）. Compared with group PH, the percentage of time of staying at the target quadrant and the number of crossing the platform within 1 min were significantly increased, the serum concentrations of TNF-α, IL-1β and HMGB1 were decreased, and the expression of hippocampal TNF-α, IL-1β and HMGB1 was down-regulated in group PH-H（P〈0.05）.Conclusion Hydrogen can improve the postoperative cognitive function in aged mice.