摘要
目的 评价七氟醚预处理对大鼠肾缺血再灌注时热休克蛋白70（HSP70）表达的影响。方法 清洁级健康成年雄性SD大鼠24只，体重250～300 g，2～3月龄，采用随机数字表法分为3组（n＝8）：假手术组（S组）、肾缺血再灌注组（I/R组）和七氟醚预处理组（SP组）。采用切除右肾后夹闭左侧肾动脉45 min再灌注2 h的方法制备肾缺血再灌注损伤模型；SP组吸入2.2%七氟醚1 h，洗脱10 min后制备肾缺血再灌注损伤模型。于再灌注2 h时取肾组织，采用免疫组化法测定HSP70的表达；采用硫代巴比妥酸比色法测定丙二醛（MDA）含量。结果 与S组比较，I/R组和SP组肾组织HSP70表达上调，肾组织MDA含量升高（P〈0.05）；与I/R组比较，SP组肾组织HSP70表达上调，肾组织MDA含量降低（P〈0.05）。结论 七氟醚预处理减轻大鼠肾缺血再灌注损伤的机制与上调HSP70表达有关。
Objective To evaluate the effect of sevoflurane preconditioning on the expression of heat shock protein 70（HSP70）during renal ischemia-reperfusion in rats.Methods Twenty-four pathogen-free healthy adult male Sprague-Dawley rats, weighing 250-300 g, aged 2-3 months, were divided into 3 groups（n=8 each）using a random number table： sham operation group（group S）, renal I/R group（group I/R）and sevoflurane preconditioning group（group SP）. The animals were anesthetized with 2% pentobarbital sodium 50 mg/kg.After the right kidney was removed, the left renal artery was clamped for 45 min followed by 2 h reperfusion to establish the model of renal I/R injury.In group SP, 2.2% sevoflurane was inhaled for 1 h followed by 10-min washout before the model was established.The animals were sacrificed at the end of reperfusion, and kidneys were then removed for determination of the expression of HSP70（by immunohistochemistry）and malondialdehyde（MDA）content in renal tissues（by thiobarbituric acid colorimetric method）.Results Compared with group S, the expression of HSP70 in renal tissues was significantly up-regulated, and the MDA content in renal tissues was increased in I/R and SP groups（P〈0.05）. Compared with group I/R, the expression of HSP70 in renal tissues was significantly up-regulated, and the MDA content in renal tissues was decreased in group SP（P〈0.05）.Conclusion The mechanism by which sevoflurane preconditioning reduces renal I/R injury is related to up-regulation of HSP70 expression in rats.
出处
《中华麻醉学杂志》
CSCD
北大核心
2017年第5期629-631,共3页
Chinese Journal of Anesthesiology
