靶向PPARγ的miRNA在冠脉支架内再狭窄中的表达及意义

Expression and significance of the miRNAs target to PPARγ in coronary artery instent restenosis

目的探索靶向于过氧化体增殖物激活型受体γ(PPARγ)的miRNA,在这些miRNA中寻找与支架内再狭窄(ISR)密切相关的miRNA,并初步探索其临床意义。方法利用Cytoscape及其插件构建miRNA与PPARγ的作用网络,筛选出靶向PPARγ的关键miRNA。利用实时荧光定量聚合酶链反应(Real-time PCR)检测这部分miRNA在ISR人群中的表达差异,再通过ROC曲线评估miRNA对ISR患者的临床识别能力。结果通过生物信息学分析发现,miR-27a/b对PPARγ的调控作用较强;而miR-130a/b则能调控该网络中更多的基因。Real-time PCR发现miR-27a/b和miR-130a在ISR组较non-ISR组存在差异性表达(P<0.05)。ROC曲线分析发现,miR-27a对ISR具有一定的临床识别能力(AUC=0.884,95%CI 0.768~1.00,P<0.001)。结论 miR-27a/b和miR-130a/b是靶向于PPARγ的主要miRNA,miR-27a/b和miR-130a在ISR中差异性表达,miR-27a可能对识别ISR患者具有一定的临床意义。

Aim To explore the key members of the miRNAs target to peroxisome proliferator-activated receptor γ（PPARγ）,and reveal the clinical significance of which are closely related to in-stent restenosis （ISR）.Methods Cytoscape and its plug-in was used to build the miRNAs-PPAR regulatory network to screen out which are the key miRNAs target to PPARγ.The expression of miRNAs in ISR group were detected by Real-time PCR and the clinical recognition ability of miRNAs on ISR patients were evaluated by ROC curve.Results By bioinformatics analysis,miR-27a/b has a strong regulatory effect on PPARγ,while miR-130a/b is able to regulate more genes in the network.Real-time PCR found that miR-27a/b and miR-130a were differentially expressed in the ISR group and no-ISR group （P〈0.05）.ROC curve analysis showed that miR-27a has a certain clinical recognition ability to ISR （AUC =0.884,95% CI 0.768～1.00,P〈0.001）.Conclusion MiR-27a/b and miR-130a/b are key members of the miRNAs target to PPARγ,miR-27a/b and miR-130a were differentially expressed in ISR,miR-27a might have certain clinical diagnostic value in patients with ISR.