载脂蛋白M基因rs707921位点多态性与冠心病易感性的关系

Association between apolipoprotein M gene rs707921 site polymorphism and susceptibility to coronary heart disease

目的检测载脂蛋白M(ApoM)基因rs707921位点的基因多态性,探讨其与冠心病(CHD)的相关性。方法采用单荧光标记探针技术检测111例CHD患者及248例对照组的ApoM rs707921位点单核苷酸多态性,分析其基因型和等位基因频率的分布情况。结果 ApoM rs707921位点3种基因型(AA型、AC型和CC型)在CHD组中分布频率为1.8%、13.5%和84.7%,在对照组中分布频率为2.0%、25.4%和72.6%,两组差异有统计学意义(P=0.039);ApoM rs707921位点A、C等位基因的频率在CHD组和对照组中分布频率分别为8.6%、91.4%和14.7%、85.3%,两组比较差异有统计学意义(P=0.023)。CHD组中AC+AA基因型者甘油三酯水平显著低于CC基因型者(P=0.043)。CHD组rs707921位点多态性不同基因型间冠状动脉病变严重程度差异无统计学意义(P>0.05)。结论ApoM基因rs707921位点A等位基因可能降低CHD的发病风险,但与CHD的严重程度无关。

Aim To investigate the association between apolipoprotein （ApoM） gene and coronary heart disease （CHD） by detecting the polymorphism of ApoM rs707921 site. Methods The polymorphism of ApoM rs707921 site was detected by single fluorescent labeling probe technique in 111 cases of CHD patients and 248 cases of control group. The distribution of genotype and allele frequency was analyzed. Results The frequencies of ApoM rs707921 genotype （AA, AC and CC） were 1.8%, 13.5% and 84.7% in CHD group, 2.0%, 25.4% and 72.6% in control group, respective- ly, there was significant difference between the two groups （P = 0.039）. The frequencies of A and C allele in ApoM rs707921 site were 8.6% and 91.4% in CHD group, 14.7% and 85.3% in control group, respectively, the difference between the two groups was statistically significant （P = 0.023）. Triglyceride level of AC＋AA genotype was significantly lower than that of CC genotype in CHD group （P = 0.043）. There was no significant difference in the severity of coronary artery lesion between different genotypes of rs707921 polymorphism in CHD group （P〉0.05）. Conclusion A allele of ApoM gene rs707921 site may reduce the risk of CHD, but has nothing to do with the severity of coronary heart disease.