基因组学研究多药耐药鲍曼不动杆菌生物被膜的基因特征

Genomics study on the genetic characteristics of multidrug resistant acinetobacter baumannii biofilm

目的 基因组学研究细菌生物被膜独特的存在方式和独特的基因表达特征。方法 选取多药耐药鲍曼不动杆菌菌株,研究同一菌株在不同生长状态、浮游细菌状态和生物被膜状态下,两个样本间基因组的表达差异;分析细菌生物被膜状态下独特的基因表达。结果 我们发现,不同生长状态下两个样本间存在基因表达差异。相对于浮游状态菌,生物被膜菌株有198个基因出现表达差异; 其中106个基因表达上调,92个基因表达下调。结论 细菌生物被膜状态下的菌体,有着自己独特的基因和蛋白质表达。菌体生理生化特性、致病力和对药物敏感性都与浮游菌状态显著不同。这些表达差异的基因中,主要集中于外膜蛋白和调控因子相关基因的表达。

Objective To study the unique presence of bacterial biofilm and unique gene expression characteristics. Methods We collected the multidrug resistant acinetobacter baumannii strains. Then we studied the same strain, but different growth status, analyzed the different gene expression. Results The whole genome sequencing of the acinetobacter baumannii did have significant difference in the expression between the two. There were significant differences in the expression of 198 genes, among which 106 genes were up-regulated and 92 genes were down-regulated in the biofilm compared to the rapidly growing cells. Conclusions Bacterial biofilm had its own unique gene and protein expressions. The physiological and biochemical characteristics, pathogenicity and drug sensitivity of the ceils were significantly different from those of the planktonic cells. These genes differ in the expression of genes, mainly focused on the outer membrane protein and regulatory factors related genes.