甜高粱茎秆总RNA提取方法的比较

Comparison and Analysis of the Methods of RNA Isolation From Sweet Sorghum Stems

甜高粱是最具潜力的能源作物之一,新鲜的甜高粱茎秆中含有大量汁液且富含多糖和多酚成分,很难获得高质量的总RNA。以甜高粱Rio的茎秆为实验材料,利用Trizol法、SDS法、改良CTAB法和RNAiso法分别提取新鲜茎秆总RNA,比较几种方法获取的总RNA质量与纯度。实验结果证明,RNAiso改良法提取的茎秆总RNA质量高,完整性好,总RNA产物可直接用于建库和转录组测序等后续实验。

Sweet sorghum is a kind of promising energy crops. There are large amounts of juice aysaccharides and polyphenols in fresh stem of sweet sorghum, so it is very difficult to oRNA by common RNA extraction methods from the fresh stem. In this study, the total R N A of Rio stem wasextracted by Trizol method, S D S method, modified C T A B method and RNAiso method respectiveshowed that the total RNA extracted usingRNAiso method is of high quality and good integrity, directly for subsequent experiments such as database construction and transcriptome sequencing.