双藤痹痛凝胶膏剂对胶原诱导性关节炎模型大鼠炎性细胞因子表达的影响

Effect of Shuangteng Bitong Cataplasm on Inflammatory Cytokines in Rats with Collagen-induced Arthritis

目的:探讨双藤痹痛凝胶膏剂对胶原诱导性关节炎模型(collagen-induced arthritis,CIA)大鼠滑膜组织中炎性细胞因子表达的影响,探讨其治疗作用机理。方法:Wistar大鼠40只,随机分成4组,分别为正常组、模型对照组、双藤痹痛凝胶高、低剂量组,通过建立CIA大鼠模型,采用酶联免疫吸附测定法(enzyme-linked immunosorbent assay,ELISA)检测血清中肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β及IL-6的含量,逆转录-聚合酶链式扩增反应(Reverse Transcription-Polymerase Chain Reaction,RT–PCR)法测定滑膜组织中TNF-α、IL-1β及IL-6 mRNA的表达水平。结果:ELISA法检测结果显示,与模型组比较,双藤痹痛凝胶高、低剂量组大鼠血清中TNF-α、IL-1β及IL-6?的含量显著下降(P<0.05或P<0.01);RT–PCR检测显示,与模型组比较,双藤痹痛凝胶膏剂高、低剂量组大鼠滑膜组织中TNF-α、IL-1β及IL-6 mRNA的表达明显降低。结论:双藤痹痛凝胶膏剂对胶原诱导性关节炎大鼠有一定的治疗作用,其机制可能与降低血清及滑膜组织中TNF-α、IL-1β及IL-6水平有关。

Objective: To study the effect of Shuangteng Bitong Cataplasm on inflammatory cytokines in rats with collagen-induced arthritis. Methods: Forty Wistar rats were randomly divided into 4 groups,normal control group,model control group,high-dose and low-dose Shuangteng Bitong Cataplasm group. Rats' arthritis was induced by collagen. The content of TNF-α,IL-1β and IL-6 in serum were evaluated by ELISA,and the expression levels of TNF-α,IL-1β and IL-6 in synovial tissues were evaluated by RT –PCR. Results: ELISA assay showed: compared with the model groups,the content of TNF-α,IL-1β and IL-6 in serum was decreased remarkably in the low and high-dose of Shuangteng Bitong Cataplasm groups.( P < 0. 05 or P < 0. 01),and RT – PCR indicated: the expression levels of TNF-α,IL-1β and IL-6 in synovial tissues was decreased remarkably in the low and high-dose of Shuangteng Bitong Cataplasm groups. Conclusion: Shuangteng Bitong Cataplasm has a certain protective effect on CIA rats and its mechanism is possibly relevant with the down-regulation of the expressions of TNF-α,IL-1β and IL-6 mRNA.