西藏藏族人群脂代谢相关基因多态性与血浆三酰甘油水平的关系研究

Relationship between Lipid Metabolism Related Gene Polymorphism and Plasma TG Level in Zang Population in Tibet

目的探究西藏藏族人群血脂相关基因多态性(SNP)与血浆三酰甘油(TG)水平的关系。方法 2008年6—8月,采用随机整群抽样法抽取西藏那曲地区安多县扎仁镇(海拔4 800 m)、日喀则地区定日县扎西宗乡(海拔4 300 m)、山南地区琼结县雪巴乡(海拔3 740 m)、拉萨市城关区蔡公堂乡(海拔3 649 m)和林芝地区林芝县布久乡(海拔3 000 m)共5个不同海拔的乡镇,按照户籍信息纳入2 348藏族居民,其中1 085例采集到血液样本。采用美国西昆诺姆公司Mass ARRAY实验平台检测脂代谢相关基因SNP。结果共选出10个脂代谢相关基因位点,包括rs964184、rs12678919、rs1260326、rs2954029、rs714052、rs7557067、rs17216525、rs10889353、rs854560、rs505151,除rs854560位点基因分型失败外,rs964184、rs12678919、rs1260326、rs2954029、rs714052、rs7557067、rs17216525、rs10889353及rs505151位点基因型分布均符合哈迪-温伯格遗传平衡。rs12678919、rs1260326、rs2954029、rs7557067、rs17216525、rs10889353及rs505151位点不同基因型藏族居民血浆TG水平比较,差异均无统计学意义(P>0.05)。rs964184位点GC和GG基因型藏族居民血浆TG水平高于CC基因型藏族居民,GG基因型藏族居民血浆TG水平高于GC基因型藏族居民(P<0.05);rs714052位点TC和TT基因型藏族居民血浆TG水平高于CC基因型藏族居民,TT基因型藏族居民血浆TG水平高于TC基因型藏族居民(P<0.05)。调整年龄、体质指数后,rs964184位点GC和GG基因型藏族居民血浆TG水平高于CC基因型藏族居民,GG基因型藏族居民血浆TG水平高于GC基因型藏族居民(P<0.05);rs714052位点TC、TT基因型藏族居民血浆TG水平高于CC基因型藏族居民,TT基因型藏族居民血浆TG水平高于TC基因型藏族居民(P<0.05)。结论西藏藏族人群脂代谢相关基因rs964184、rs714052位点SNP与血浆TG水平有关。

objective To study the relationship between lipid metabolism related gene polymorphism and plasma TG level in Zang population in Tibet. Methods From June to August in 2008 in Tibet, a total of 5 towns locating with different altitude were selected by random cluster sampling method, including Zharen Town of Amdo County of Nagqu Prefecture （altitude ： 4800 m） , Tashi Town of Tingri County of Shigatse Prefecture （ altitude ： 4 300 m ） , Sherpa Town of Qonggyai County of Lhoka Prefecture （ altitude ： 3 740 m） , Choi Court Town of Chengguan District of Chengguan District （ altitude ： 3 649 m） and Puqu Town of Nyingchi County of Nyingchi Prefecture （ altitude ： 3 000 m） , and then 2 348 Tibetan residents were enrolled according to household registration information, thereinto 1 085 Tibetan residents＇ blood samples were collected. MassARRAY experiment platform （ Sequenom, Inc） was used to detect the lipid metabolism related gene polymorphism. Results A total of 11 lipid metabolism related genetic locus were found, including rs964184, rsl2678919, rsl260326, rs2954029, rs714052, rs7557067, rsl7216525, rsl0889353, rs854560, rs505151, genotype distribution of above lipid metabolism related genetic locus was in accord with Hardy - Weinberg genetic equilibrium, respectivelly, excepted for rs854560. No statistically significant differences of plasma TG level was found in Tibetan residents with different genotypes of rsl2678919, rsl260326, rs2954029, rs7557067, rsl7216525, rsl0889353 or rs505151 （P〉0.05）. Plasma TG level of Tibetan residents with GC or GG genotype of rs964184 was statistically significantly higher than that of Tibetan residents with CC genotype of rs964184, respectively, meanwhile plasma TG level of Tibetan residents with GG genotype of rs964184 was statistically significantly higher than that of Tibetan residents with GC genotype of rs964184 （P 〈 0. 05 ） . Plasma TG level of Tibetan residents with TC or TT genotype of rs714052 was statistically significantly higher than that of Tibetan residents with CC genotype of rs714052, respectively, meanwhile plasma TG level of Tibetan residents with TT genotype of rs714052 was statistically significantly higher than that of Tibetan residents with TC genotype of rs714052 （ P 〈 0. 05 ） . After correction of age and BMI, plasma TG level of Tibetan residents with GC or GG genotype of rs964184 was statistically significantly higher than that of Tibetan residents with CC genotype of rs964184, respectively, meanwhile plasma TG level of Tibetan residents with GG genotype of rs964184 was statistically significantly higher than that of Tibetan residents with GC genotype of rs964184 （ P 〈0. 05） ; plasma TG level of Tibetan residents with TC or TT genotype of rs714052 was statistically significantly higher than that of Tibetan residents with CC genotype of rs714052, respectively, meanwhile plasma TG level of Tibetan residents with TT genotype of rs714052 was statistically significantly higher than that of Tibetan residents with TC genotype of rs714052 （ P 〈 0. 05 ） .Conclusion Lipid metabolism related gene polymorphism （including rs964184 and rs714052） is significantly correlated with plasma TG level in Zang population in Tibet.