辣椒素诱导人骨肉瘤细胞MG-63发生免疫原性细胞死亡

Immunogenic cell death of human osteosarcoma cells MG-63 induced by capsaicin

目的探讨辣椒素与顺铂能否抑制人骨肉瘤细胞MG-63增殖并诱导其免疫原性死亡(ICD)。方法 MTT检测细胞的增殖;线粒体膜电位分析细胞凋亡;流式细胞计量术检测细胞膜上钙网蛋白(CRT)表达量;荧光素酶法检测细胞外ATP的释放;ELISA检测细胞上清中高迁移率族蛋白1(HMGB1)的分泌。结果辣椒素及顺铂作用均能抑制细胞MG-63增殖(P<0.01),且呈剂量依赖性;辣椒素和顺铂均可诱导MG-63细胞凋亡(P<0.01),但只有辣椒素能诱导MG-63细胞内质网中CRT转移到细胞膜表面,且促进ATP及HMGB1的释放(P<0.01)。结论辣椒素可诱导人骨肉瘤细胞凋亡及免疫原性死亡。

Objective To investigate the effect of capsaicin and cisplatin on the proliferation and immunogenic cell death of human osteosarcoma cells. Methods MTT assay was used to examine the growth inhibiting effects of capsacin and cisplation on MG-63 cells; Mitochondrial membrane potential （MMP） was used to investigate the apoptosis; flow cytometry was used to detect the expression of calreticulin（CRT） on the cell membrane, fluorescein enzymatic method was used to detect the release of ATP and ELISA was used to detect the secretion of high mobility group B1 （HMGB1）. Results Capsaicin and cisplatin can inhibit MG-63 cells proliferation in the dose-dependent manner and induce MG-63 apoptosis（P＜0.01）. Only capsaicin can induce translocation of CRT from endoplasmic reticulum to the cell surface and release of extracellular ATP and HMGB1（P＜0.01）. Conclusions Capsaicin can induce human osteosarcoma cells apoptosis and immunogenic cell death.