腹腔热灌注与顺铂联用增加人卵巢癌细胞系凋亡

Combination of hyperthermic intraperitoneal perfusion and cisplatin increases apoptosis in human ovarian cancer cell lines

目的研究腹腔热灌注(HIPE)和顺铂对人卵巢癌细胞凋亡的影响。方法将人卵巢癌OVCAR-3和A2780细胞各分为对照组、顺铂组、热疗组和热化疗组;显微镜观察卵巢癌细胞形态;AO/GV染色、流式细胞仪检测卵巢癌细胞凋亡细胞比率;荧光定量PCR(q-PCR)检测卵巢癌细胞凋亡相关基因caspase7、caspase9和Bid等。结果 1)顺铂组、热疗组及热化疗组卵巢癌细胞回缩,部分漂浮,其中热化疗组细胞变化最明显;2)顺铂组、热疗组及热化疗组较对照组凋亡细胞增多,其中热化疗组凋亡细胞数量最多(P<0.05);3)顺铂组、热疗组、热化疗组较对照组凋亡细胞比率增多,热化疗组凋亡细胞比率最大(P<0.05);4)促凋亡相关基因caspase3、caspase6、caspase7、caspase8、caspase9、Bax、Bak和Bid在热化疗组表达明显高于其他各组,凋亡抑制相关基因Bcl-2、Bcl-x L、Mcl-1和c-FLIP表达较其他各组明显下降。结论顺铂和热疗可促进卵巢癌细胞凋亡,且热化疗联合作用效果最明显。

Objective To investigate the effect of hyperthermic intraperitoneal perfusion and cisplatin on apoptosis in human ovarian cancer cells. Metheds Two human ovarian cancer cells （OVCAR-3, A2780） were divided into control group, cisplatin group, hyperthermia group and thermo-chemotherapy group; microscope was used to observe the morphological changes of the four groups; AO/GV stain and flow cytometry（FCM） was used to analyse cell apoptosis; Detection of apoptosis related genes CASPASE7、 CASPASE8 and BAX in ovarian cancer cells by fluorescence quantitative PCR. Results Inverted microscope observeed that the ovarian cancer cells were retracted and suspended partially in the cisplatin group and hyperthermia group, especially in the thermo-chemotherapy group. After AO/GV staining, the apoptotic cells were increased in the cisplatin group and hyperthermia group compared with the control group, and the thermo-chemotherapy group is more than cisplatin group and hyperthermia group. FCM results indicated that the proportion of cells apoptosis were higher in the cisplatin group and hyperthermia group, the thermo-chemotherapy group is the highest than the other groups（p〈0.05）. q-PCR results showed that in the thermo-treatment group the expression of pro-apoptotic genes, including CASPASE3、CASPASE6、CASPASE7、CASPASE8、CASPASE9，BAX、BAK and BID, were significantly higher than other groups, apoptosis inhibitory gennes, such as BCL-2、BCL-XL、MCL-1、c-FLIP, were significantly decreased than the others. Conclusion Cisplatin and hyperthermia can promote the apoptosis in ovarian cancer cells, especially by the thermo-chemotherapy.