内蒙古奇乾地区蜱携带斑点热立克次体的调查

Investigation on spotted fever group Rickettsiae in ticks collected from Qiqian areas of Inner Mongolia

目的了解内蒙古奇乾地区媒介蜱携带斑点热立克次体(spotted fever group Rickettsiae,SFGR)的感染情况。方法2014年4月,在内蒙古奇乾地区用布旗法采集游离蜱,用PCR和序列测定方法对媒介蜱样本中SFGR的感染进行检测和基因分型。结果共采集媒介蜱320只,其中全沟硬蜱293只(91.56%)、森林革蜱22只(6.88%)、嗜群血蜱5只(1.56%)。媒介蜱SFGR总阳性率为47.50%,其中全沟硬蜱阳性率为46.42%,携带新塔拉塞维奇立克次体(Candidatus Rickettsia tarasevichia,CRT)、拉欧蒂立克次体(Rickettsia raoultii,R.raoultii)和黑龙江立克次体(Rickettsia heilongjiangii,R.hei)3种基因型;森林革蜱阳性率为59.09%,携带CRT和R.raoultii 2种基因型,嗜群血蜱阳性率60.00%,携带R.hei基因型。结论内蒙古奇乾地区全沟硬蜱为优势蜱种,媒介蜱中携带SFGR基因型存在多样性,应引起公共卫生部门的高度重视。

Objective To detect the types of spotted fever group Rickettsiae （SFGR） in ticks collected from Qiqian areas of Inner Mongolia. Methods April 2014,with the dragging method, free ticks were collected in Qiqian areas of Inner Mongolia,PCR was used to study the infection and genotype of SFGR in tick samples. Results A total of 320 ticks were collected in Qiqian areas,including lxodes persulcatus 293 （46.42%）,Dermacentor silvarum 22（6. 88%）,Haemaphysalis conieinna 5（2.65%）. The positive rate of SFGR was 47.50%. In Ixodes persulcatus,Dermacentor silvarum,Haemaphysalis conicinna was 46.42% ,59.09% ,60.00% ,respectively. Genotyping results showed that there were three genotypes in ticks：Candidatus Rickettsia tarasevichiae （CRT）,Rickettsia raoultii（R.raoultii） and Rickettsia heilongjiangii（R.helongfiangii）. Conclusion Ixodes persulcatus was the dominant species in Qiqian areas,and various kinds of genotypes of SFGR existed in the ticks,which should be paid attention to by local public health authorities.