流式细胞术检测血小板相关抗体在46例维吾尔族免疫性血小板减少症患者中的应用

Application of flow cytometry in detection of platelet associated antibodies in 46 patients with immune thrombocytopenic purpura

目的分析探讨流式细胞术检测血小板相关抗体在维吾尔族免疫性血小板减少症中的临床应用价值。方法将来该院就诊的维吾尔族免疫性血小板减少症患者46例设为观察组,将在该院健康体检的维吾尔族健康者46例设为对照组,两组研究对象均采取流式细胞术对血小板相关抗体进行检测,分析观察组与对照组之间血小板相关抗体的表达率和荧光强度。结果观察组PAIg G、PAIg A和PAIg M的阳性率分别为80.43%、76.09%和78.26%,均显著高于对照组的54.35%、54.35%和54.35%(P<0.05);观察组的巨核细胞和血小板的数量分别为(58±11)个和(223±35)×10~9/L,均明显多于对照组的(23±7)个和(57±23)×10~9/L(P<0.05)。结论流式细胞术可快速检出免疫性血小板减少症患者中相关的抗体变化,其在临床检测中具有应用意义。

[Objective] To explore the clinical value of flow cytometric detection of platelet associated antibody in Uygur immune thrombocytopenia. [Methods] Forty-six Uygur patients with immune thrombocytopenia admitted in our hospital were included in the observation group, 46 healthy subjects undergoing the health examination in the hospital were included in the control group, platelet related antibodies of subjects in the two groups were detected by flow cytometry, and the differences in the expression rate and fluorescence intensity ofplatelet related antibodies between observation group and control group were compared. [Results] The positive rates of platelet associated immunoglobulin G （PAIgG）, platelet associated immunoglobulin A （PAIgA） and platelet associated immunoglobulin M （PAIgM） in the observation group were 80.43%, 76.09% and 78.26%, which were significantly higher than 54.35%, 54.35% and 54.35% in the control group （P 〈0.05）; the number of megakaryocytes and platelets in the observation group were （58 ±11） and （223± 35）×10^9/L, which were significantly higher than （23 ± 7） and （57± 23） ×10^9/L in the control group （P 〈0.05）. [Conclusion] Flow cytometry can rapidly detect related antibody changes in patients with immune thrombocytopenia, which has application significance in clinical detection.